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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [6]
- Immunohistochemistry [5]
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- Product number
- MA5-24642 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GRASP55 Monoclonal Antibody (CL2610)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SGELLSSLPP TSNAPSDPAT TTAKADAASS LTVDVTPPTA KAPTTVEDRV GDSTPVSEKP VSAAVDANAS ESP Binds to an epitope located within the peptide sequence DAASSLTVDVTPPTA as determined by overlapping synthetic peptides.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CL2610
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GRASP55 in Lane1: Marker (kDa), Lane 2:Human cell line U-251 MG. Samples were probed using a GRASP55 Monoclonal Antibody (Product # MA5-24642).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GRASP55 in Lane1: Marker (kDa), Lane 2: Human cell line HeLa cytoplasmic fraction, Lane 3: Human cell line HeLa membrane fraction, Lane 4: Human cell line HeLa nuclear fraction, Lane 5: Human cell line HeLa chromatin fraction, Lane 6: Human cell line HeLa cytoskeletal fraction. Samples were probed using a GRASP55 Monoclonal Antibody (Product # MA5-24642).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using GRASP55 Monoclonal Antibody (CL2610) (Product # MA5-24642). Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of GRASP55 in U251 cell line using a GRASP55 Monoclonal Antibody (Product # MA5-24642), showing specific staining of the golgi apparatus in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of GRASP55 in HeLa cell line using a GRASP55 Monoclonal Antibody (Product # MA5-24642), showing specific staining of the golgi apparatus in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of GRASP55 in A431 cell line using a GRASP55 Monoclonal Antibody (Product # MA5-24642), showing specific staining of the golgi apparatus in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of GRASP55 in MCF7 cell line using a GRASP55 Monoclonal Antibody (Product # MA5-24642), showing specific staining of the golgi apparatus in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of GRASP55 in U2OS cell line using a GRASP55 Monoclonal Antibody (Product # MA5-24642), showing specific staining of the golgi apparatus in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of GRASP55 in U251 cell line using a GRASP55 Monoclonal Antibody (Product # MA5-24642), showing specific staining of the golgi apparatus in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GRASP55 in human epididymis and skeletal muscle tissues using GRASP55 Monoclonal Antibody (CL2610) (Product # MA5-24642). Corresponding GORASP2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GRASP55 in human endometrium using GRASP55 Monoclonal Antibody (CL2610) (Product # MA5-24642) shows moderate to strong membranous and cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GRASP55 in human epididymis using GRASP55 Monoclonal Antibody (CL2610) (Product # MA5-24642) shows strong positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GRASP55 in human prostate using GRASP55 Monoclonal Antibody (CL2610) (Product # MA5-24642) shows moderate to strong membranous positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GRASP55 in human skeletal muscle using GRASP55 Monoclonal Antibody (CL2610) (Product # MA5-24642) shows no positivity in striated muscle fibers as expected.
