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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- LS-B12071 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ Complement C1s Antibody (clone 4D9) LS-B12071
- Antibody type
- Monoclonal
- Description
- Protein A/G purified
- Reactivity
- Human, Mouse, Rat, Canine, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4D9
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C1S (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C1S.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of extracts (35 ug) from 9 different cell lines by using anti-C1S monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
