679604

antibody from BioLegend

Targeting: IGF2BP2 IMP-2

Immunocytochemistry (Data presented on provider website)

Immunoelectron microscopy (Supportive data in Antibodypedia)

Antibody Data

Product number

679604

Provider

BioLegend

Proper citation

BioLegend Cat#679604, RRID:AB_2572061

Product name

Alexa Fluor® 594 anti-IGF2BP2

Antibody type

Monoclonal

Antigen

Full length recombinant human IGF2BP2 protein (NP_006539) produced in HEK 293T cells.

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

O93C8

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunoelectron microscopy

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

HeLa cells were fixed with 2% paraformaldehyde (PFA) for ten minutes and then were permeabilized with 0.5% Triton X-100 for five minutes followed by blocking with 5% FBS for 30 minutes. The cells were then intracellularly stained with 2.5 µg/ml Alexa Fluor® 594 anti-IGF2BP2 antibody (clone O93C8) (red) in a blocking buffer overnight at 4°C, followed by Alexa Fluor® 488 phalloidin (green) staining for 20 minutes at room temperature. Nuclei were counterstained with DAPI (blue).

Conjugate

Yellow dye