GTX10194

antibody from GeneTex

Targeting: PAPPA ASBABP2, DIPLA1, IGFBP-4ase, PAPA, PAPP-A, PAPPA1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

GTX10194

Provider

GeneTex

Proper citation

GeneTex Cat#GTX10194, RRID:AB_380920

Product name

PAPP A antibody [3C8]

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Detection of human PAPP-A and proMBP subunits of htPAPP-A by monoclonal antibodies in Western blot. Lane 1: PAPP A antibody [18A10] (GTX10196); Lane 2: PAPP A antibody [3C8] (GTX10194); Lane 3: PAPP A antibody [5H9] (GTX10191); Lane 4: PAPP A antibody [11E4] (GTX14597); Lane 5: PAPP A antibody [10A5] (GTX10193); Lane 6: PAPP A antibody [3C8] (GTX10194); Lanes 1-4: reducing conditions; Lanes 5-6: non-reducing conditions, heterotetrameric complex was detected by PAPP A antibody [10A5] (GTX10193) and PAPP A antibody [3C8] (GTX10194)

ELISA

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Calibration curve for PAPP-A sandwich immunoassay. Capture: PAPP A antibody [4G11] (GTX10192) biotinylated; Detection: PAPP A antibody [3C8] (GTX10194) labeled with stable Eu3+ -chelate; Antigen: htPAPP-A; Mixture of antibodies and antigen was incubated for 30 minutes at room temperature in streptavidin-coated plates.