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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Other assay [3]
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- Product number
- PA5-29468 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Fetuin B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, mouse plasma, rat plasma. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.31 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Oviductal estrogen receptor α signaling prevents protease-mediated embryo death.
Winuthayanon W, Bernhardt ML, Padilla-Banks E, Myers PH, Edin ML, Lih FB, Hewitt SC, Korach KS, Williams CJ
eLife 2015 Dec 1;4:e10453
eLife 2015 Dec 1;4:e10453
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Fetuin B was performed in SK N_SH cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Fetuin B Polyclonal Antibody (Product # PA5 29468) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Fetuin B was performed in SK-N-SH whole cell extracts using 5 µg of Fetuin B Polyclonal Antibody (Product # PA5-29468). Samples were transferred to a membrane and probed with Fetuin B Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5--figure supplement 1. Comparable expression of proteases and protease inhibitors in wild-type (WT) and mesenchymal conditional knockout (cKO) oviduct at 0.5 dpc. ( A ) Real-time PCR of the indicated proteases and protease inhibitors in WT and mesenchymal cKO oviducts at 0.5 dpc (n = 4-6 mice/group; mean +- SEM). ( B ) Immunoblot analysis and ( C ) normalized signal intensities of fetuin B protein to beta-actin in WT and mesenchymal cKO oviducts at 0.5 dpc. dpc: Days post coitum. DOI: http://dx.doi.org/
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5. Alterations in expression of proteases and protease inhibitors in oviducts lacking epithelial estrogen receptor alpha (ERalpha). Real-time PCR of the indicated ( A ) proteases and ( B ) protease inhibitors in wild-type (WT) and conditional knockout (cKO) oviducts at 0.5 dpc (n = 4-7 mice/group; mean +- SEM). ( C ) Immunoblot analysis of fetuin B in WT and cKO oviducts; beta-actin served as a loading control. Protein lysate from one mouse in each lane; n = 4-5 mice/group. ( D ) Quantitation of fetuin B signal intensity normalized to beta-actin. ( E ) Fetuin B localization in WT and cKO oviducts at 0.5 dpc. Images shown are representative of n = 4 mice/group. Scale bar = 50 mum. For all panels, asterisk indicates significant difference compared to WT, p
