10414-1-AP

antibody from Proteintech Group

Targeting: BCAS2 DAM1, Snt309, SPF27

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

10414-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#10414-1-AP, RRID:AB_2063400

Product name

BCAS2 antibody

Antibody type

Polyclonal

Description

KD/KO validated BCAS2 antibody (Cat. #10414-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat, monkey and has been validated for the following applications: IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat, Simian

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

BCAS2 Participates in Insulin Synthesis and Secretion via mRNA Alternative Splicing in Mice.

Chen X, Xie X, Li J, Sun L, Lv Z, Yao X, Li L, Jin H, Cui S, Liu J
Endocrinology 2023 Nov 20;165(1)

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BCAS2 is involved in alternative splicing and mouse oocyte development.

Zhang J, Liu W, Li G, Xu C, Nie X, Qin D, Wang Q, Lu X, Liu J, Li L
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022 Feb;36(2):e22128

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Breast carcinoma-amplified sequence 2 regulates adult neurogenesis via β-catenin.

Chen HH, Lu HY, Chang CH, Lin SH, Huang CW, Wei PH, Chen YW, Lin YR, Huang HS, Wang PY, Tsao YP, Chen SL
Stem cell research & therapy 2022 Apr 11;13(1):160

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BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis.

Liu W, Wang F, Xu Q, Shi J, Zhang X, Lu X, Zhao ZA, Gao Z, Ma H, Duan E, Gao F, Gao S, Yi Z, Li L
Nature communications 2017 Jan 27;8:14182

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BCAS2 interacts with HSF4 and negatively regulates its protein stability via ubiquitination.

Liao S, Du R, Wang L, Qu Z, Cui X, Li C, Liu F, Huang M, Wang J, Chen J, Gao M, Yu S, Tang Z, Li DW, Jiang T, Liu M
The international journal of biochemistry & cell biology 2015 Nov;68:78-86

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BCAS2 promotes prostate cancer cells proliferation by enhancing AR mRNA transcription and protein stability.

Kuo PC, Huang CW, Lee CI, Chang HW, Hsieh SW, Chung YP, Lee MS, Huang CS, Tsao LP, Tsao YP, Chen SL
British journal of cancer 2015 Jan 20;112(2):391-402

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 10414-1-AP(BCAS2 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using BCAS2 antibody 10414-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-BCAS2 (IP:10414-1-AP, 3ug; Detection:10414-1-AP 1:1000) with mouse brain tissue lysate 6500ug.

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human colon cancer using 10414-1-AP(BCAS2 antibody) at dilution of 1:100 (under 25x lens)

Sample type

tissue