LF-MA0176

antibody from Invitrogen Antibodies

Targeting: GSN DKFZp313L0718

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

LF-MA0176

Provider

Invitrogen Antibodies

Product name

Anti-Gelsolin Monoclonal Antibody (35B2)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

A suggested positive control for this product is human plasma.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

35B2

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF7 (Lane 1), PANC-1 (Lane 2), T-47D (Lane 3), and human plasma (5 µL) (Lane 4). The blot was probed with Mouse Anti-Gelsolin Monoclonal Antibody (Product # LF-MA0176, 1:250 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 83 kDa band corresponding to Gelsolin was observed across the cell lines and samples tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).