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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- MA5-34684 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Gelsolin Recombinant Rabbit Monoclonal Antibody (JB36-68)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: Human spleen tissue, human kidney tissue, mouse lung tissue, NIH-3T3, SiHa, mouse thymus tissue lysate, mouse lung tissue lysate, THP-1, HepG2, THP-1.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JB36-68
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Gelsolin was performed by loading 10 µg of WT (lane 1) and GSN CRISPR KO (lane 2) U2OS cell lysates in RIPA buffer onto a 4-15% gradient polyacrylamide gel. Proteins were transferred to nitrocellulose membrane and blocked in 5% milk. Ponceau stained transfer of blot is shown. Gelsolin was detected at approximately 75 kDa using a Gelsolin recombinant monoclonal antibody (Product # MA5-34684) at a dilution of 1:10,000 in 5% BSA in TBST overnight at 4 deg, followed by secondary antibody diluted to 0.2 µg/mL using Goat anti-Rabbit IgG (H+L) HRP antibody (Product # 65-6120). Chemiluminescent detection was performed using Pierce ECL Western Blotting Substrate (Product # 32106). Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Gelsolin in Lane 1: Mouse thymus, Lane 2: Mouse lung, Lane 3: THP-1, Lane 4: HepG2. Samples were incubated with Gelsolin monoclonal antibody (Product # MA5-34684), at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Gelsolin was performed by loading 100 µg of U-2 OS wildtype (Lane 1) and U-2 OS Gelsolin knockout (Lane 2) whole cell extracts. The blot was probed with Anti-Gelsolin Recombinant Rabbit Monoclonal Antibody (JB36-68) (Product # MA5-34684, 1:1000 dilution) and HRP conjugated secondary antibody. Loss of signal upon knockout (KO) confirms that antibody is specific to Gelsolin. Data courtesy of Antibody Data Exchange Program.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Gelsolin in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Gelsolin monoclonal antibody (Product # MA5-34684), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Gelsolin in NIH-3T3 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Gelsolin monoclonal antibody (Product # MA5-34684), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Gelsolin in paraffin-embedded mouse lung tissue. Samples were incubated with Gelsolin monoclonal antibody (Product # MA5-34684), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Gelsolin in paraffin-embedded human spleen tissue. Samples were incubated with Gelsolin monoclonal antibody (Product # MA5-34684), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Gelsolin in paraffin-embedded human kidney tissue. Samples were incubated with Gelsolin monoclonal antibody (Product # MA5-34684), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Gelsolin in THP-1 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Gelsolin monoclonal antibody (Product # MA5-34684) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
