Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP1-33604 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-33604, RRID:AB_2115491
- Product name
- Rabbit Polyclonal Gelsolin/GSN Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Gelsolin/GSN Antibody [NBP1-33604] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela 7. 5% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Gelsolin/GSN Antibody [NBP1-33604] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Gelsolin/GSN Antibody [NBP1-33604] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Gelsolin/GSN Antibody [NBP1-33604] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Gelsolin/GSN Antibody [NBP1-33604] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody [NBP1-33604] - Mouse brain. Gelsolin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody [NBP1-33604] - Mouse urinary bladder dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min