PA5-19848

antibody from Invitrogen Antibodies

Targeting: GSN DKFZp313L0718

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA5-19848

Provider

Invitrogen Antibodies

Product name

Gelsolin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

For Western Blot, this antibody has non-specific bands at 120 kDa,140 kDa and 52 kDa.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.3 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Rat kidney tissue lysate using Product # PA5-19848, Gelsolin primary antibody at a dilution of 1 µg/mL (lane 1). Staining of Mouse spleen tissue lysate at a dilution of 1 µg/mL (Lane 2). Staining of Rat thymus tissue lysate at a dilution of 1 µg/mL (lane 3). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Rat kidney tissue lysate using Product # PA5-19848, Gelsolin primary antibody at a dilution of 1 µg/mL (lane 1). Staining of Mouse spleen tissue lysate at a dilution of 1 µg/mL (Lane 2). Staining of Rat thymus tissue lysate at a dilution of 1 µg/mL (lane 3). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of PC12 cells using Product # PA5-19848, anti-Gelsolin antibody. The cells were fixed with PFA (4%) for 10 minutes, permabilised with TBS-T (20mins), BSA (1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% PBS-Tween for 1 hour and exposed to the primary antibody at a concentration of 1 µg/mL overnight at 4C. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).