Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [7]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-27350 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Gelsolin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299, HeLa, human plasma, rat plasma, mouse plasma.
- Concentration
- 0.27 mg/mL
Submitted references Renal carcinoma/kidney progenitor cell chimera organoid as a novel tumorigenesis gene discovery model.
Xu Q, Junttila S, Scherer A, Giri KR, Kivelä O, Skovorodkin I, Röning J, Quaggin SE, Marti HP, Shan J, Samoylenko A, Vainio SJ
Disease models & mechanisms 2017 Dec 19;10(12):1503-1515
Disease models & mechanisms 2017 Dec 19;10(12):1503-1515
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Gelsolin using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Gelsolin polyclonal antibody (Product # PA5-27350) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Gelsolin in Human tissue extract (30 µg). Samples was separated by 7.5% SDS-PAGE and the membrane was probed with Gelsolin Polyclonal antibody (Product # PA5-27350) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Gelsolin Polyclonal Antibody (Product # PA5-27350). Sample (30 µg of whole cell lysate). Lane A: H1299. Lane B: Hela . 7.5% SDS PAGE. Gelsolin Polyclonal Antibody (Product # PA5-27350) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Gelsolin was performed by separating 50 µg of Mouse tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Gelsolin Polyclonal Antibody (Product # PA5-27350) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Gelsolin Polyclonal Antibody (Product # PA5-27350). Sample (30 µg of whole cell lysate). Lane A: H1299. Lane B: Hela . 7.5% SDS PAGE. Gelsolin Polyclonal Antibody (Product # PA5-27350) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Gelsolin Polyclonal Antibody detects Gelsolin protein by western blot analysis. Human tissue extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin Polyclonal Antibody (Product # PA5-27350) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Gelsolin was performed by separating 50 µg of Rat tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Gelsolin Polyclonal Antibody (Product # PA5-27350) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Gelsolin in methanol-fixed HeLa cells using a Gelsolin polyclonal antibody (Product # PA5-27350) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Gelsolin (Product # PA5-27350) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.