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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- AF2154 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal B7-H4 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse B7-H4 in direct ELISAs and Western blots. Detects human and mouse B7-H4 in Western blots.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Molecular Pathways: Evaluating the Potential for B7-H4 as an Immunoregulatory Target.
Loss of Peripheral Protection in Pancreatic Islets by Proteolysis-Driven Impairment of VTCN1 (B7-H4) Presentation Is Associated with the Development of Autoimmune Diabetes.
B7-H3 and B7x are highly expressed in human prostate cancer and associated with disease spread and poor outcome.
MacGregor HL, Ohashi PS
Clinical cancer research : an official journal of the American Association for Cancer Research 2017 Jun 15;23(12):2934-2941
Clinical cancer research : an official journal of the American Association for Cancer Research 2017 Jun 15;23(12):2934-2941
Loss of Peripheral Protection in Pancreatic Islets by Proteolysis-Driven Impairment of VTCN1 (B7-H4) Presentation Is Associated with the Development of Autoimmune Diabetes.
Radichev IA, Maneva-Radicheva LV, Amatya C, Salehi M, Parker C, Ellefson J, Burn P, Savinov AY
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 15;196(4):1495-506
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 15;196(4):1495-506
B7-H3 and B7x are highly expressed in human prostate cancer and associated with disease spread and poor outcome.
Zang X, Thompson RH, Al-Ahmadie HA, Serio AM, Reuter VE, Eastham JA, Scardino PT, Sharma P, Allison JP
Proceedings of the National Academy of Sciences of the United States of America 2007 Dec 4;104(49):19458-63
Proceedings of the National Academy of Sciences of the United States of America 2007 Dec 4;104(49):19458-63
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse and Human B7-H4 by Western Blot. Western blot shows lysates of mouse placenta tissue, mouse uterus tissue, and SK-BR-3 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse B7-H4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for B7-H4 at approximately 50-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of B7-H4 in HEK293 Human Cell Line Transfected with Mouse B7-H4 and eGFP by Flow Cytometry. Detection of B7-H4 in HEK293 Human Cell Line Transfected with Mouse B7-H4 and eGFP by Flow Cytometry.
