Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- MAB2154 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal B7-H4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse B7-H4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) B7-1, rmB7-2, rmB7-H1, rmB7-H2, rmB7-H3, recombinant human (rh) B7-H3b, rhB7-H4, or rmPD-L2 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Loss of Peripheral Protection in Pancreatic Islets by Proteolysis-Driven Impairment of VTCN1 (B7-H4) Presentation Is Associated with the Development of Autoimmune Diabetes.
Radichev IA, Maneva-Radicheva LV, Amatya C, Salehi M, Parker C, Ellefson J, Burn P, Savinov AY
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 15;196(4):1495-506
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 15;196(4):1495-506
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of B7-H4 in HEK293 Human Cell Line Transfected with Mouse B7-H4 and eGFP by Flow Cytometry. Detection of B7-H4 in HEK293 Human Cell Line Transfected with Mouse B7-H4 and eGFP by Flow Cytometry.