Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- FAB2154G - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse B7-H4 Alexa Fluor® 488-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse B7-H4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) B7-1, rmB7-2, rmB7-H1, rmB7-H2, rmB7-H3, recombinant human (rh) B7-H3b, rhB7-H4, or rmPD-L2 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Green dye
- Antigen sequence
Q7TSP5
- Isotype
- IgG
- Antibody clone number
- 297219
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of B7-H4 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse B7-H4 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB2154G) or (B) Rat IgG2B Alexa Fluor 488 Isotype Control (Catalog # IC013G). View our protocol for Staining Membrane-associated Proteins.