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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- V8175 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- B7-H4 Antibody / VTCN1
- Antibody type
- Monoclonal
- Description
- This highly specific B7-H4 antibody is suitable for use in ELISA/Flow cytometry/Immunofluorescence/Western blot/Immunohistochemistry applications with human samples.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- B7H4/1788
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the B7-H4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human HCT-116 cell lysate with B7-H4 antibody. Predicted molecular weight: ~31 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed human SK-BR-3 cells with B7-H4 antibody (clone B7H4/1788, green) and Reddot nuclear stain (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human testicular carcinoma with B7-H4 antibody (clone B7H4/1788). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed human SK-BR-3 cells with B7-H4 antibody (clone B7H4/1788); Red=isotype control, Blue= B7-H4 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free B7-H4 antibody (clone B7H4/1788) as confirmation of integrity and purity.
