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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- PA5-56974 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAN1A2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KLRKSREEIR AEIQTEKNKV VQEMKIKENK PLPPVPIPNL VGIRGGDPED NDIREK Highest antigen sequence identity to the following orthologs: Mouse - 73%, Rat - 75%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.40 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references N-Glycans and sulfated glycosaminoglycans contribute to the action of diverse Tc toxins on mammalian cells.
Song N, Chen L, Ren X, Waterfield NR, Yang J, Yang G
PLoS pathogens 2021 Feb;17(2):e1009244
PLoS pathogens 2021 Feb;17(2):e1009244
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MAN1A2 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4. Samples were probed using a MAN1A2 Polyclonal Antibody (Product # PA5-56974).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of MAN1A2 in human cell line A-431 shows positivity in the Golgi apparatus. Samples were probed using a MAN1A2 Polyclonal Antibody (Product # PA5-56974).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MAN1A2 in human placenta and skeletal muscle tissues using MAN1A2 Polyclonal Antibody (Product # PA5-56974). Corresponding MAN1A2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MAN1A2 in human placenta using MAN1A2 Polyclonal Antibody (Product # PA5-56974) shows strong granular cytoplasmic positivity in trophoblastic cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MAN1A2 in human prostate using MAN1A2 Polyclonal Antibody (Product # PA5-56974) shows strong granular cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MAN1A2 in human kidney using MAN1A2 Polyclonal Antibody (Product # PA5-56974) shows strong granular cytoplasmic positivity in cells in tubules.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MAN1A2 in human skeletal muscle using MAN1A2 Polyclonal Antibody (Product # PA5-56974) shows very weak positivity in myocytes.
