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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-51508 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PHKA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: AHSLRCSAEE ATEGLMNLSP SAMKNLLHHI LSGKEFGVER SVRPTDSNVS PAISIHEIGA VGATKTERTG IMQLKSEIKQ SPGTSMTPSS GSFPSAYDQQ SSKDSRQGQW Q Highest antigen sequence identity to the following orthologs: Mouse - 90%, Rat - 80%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Intracellular calcium leak lowers glucose storage in human muscle, promoting hyperglycemia and diabetes.
Tammineni ER, Kraeva N, Figueroa L, Manno C, Ibarra CA, Klip A, Riazi S, Rios E
eLife 2020 May 4;9
eLife 2020 May 4;9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PHKA1 in Lane 1: Marker (kDa) 219, 112, 85, 49, 32, 25, 18; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human cell line A-431; Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using a PHKA1 Polyclonal Antibody (Product # PA5-51508).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PHKA1 in human cell line A-431 using a PHKA1 Polyclonal Antibody (Product # PA5-51508) shows localization to vesicles.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PHKA1 in human parathyroid gland and small intestine tissues using PHKA1 Polyclonal Antibody (Product # PA5-51508). Corresponding PHKA1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PHKA1 in human parathyroid gland using PHKA1 Polyclonal Antibody (Product # PA5-51508) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PHKA1 in human small intestine using PHKA1 Polyclonal Antibody (Product # PA5-51508) shows low expression as expected.
