PA5-96759

antibody from Invitrogen Antibodies

Targeting: HOXD10 HOX4, HOX4D

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96759

Provider

Invitrogen Antibodies

Product name

HOXD10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MSFPNSSPAA NTFLVDSLIS ACRSDSFYSS SASMYMPPPS ADMGTYGMQT CGLLPSLAKR EVNHQNMGMN VHPYIPQVDS WTDPNRSCRI EQPVTQQVPT CSFTTNIKEE SNCCMYSDKR NKLISAEVPS YQRLVPESCP VENPEVPVPG YFRLSQTYAT GKTQEYNNSP EGSSTVMLQL NPRGAAKPQL SAAQLQMEKK MNEPVSGQEP TKVSQVESPE AKGGLPEERS CLAEVSVSSP; Positive Samples: Mouse kidney, Mouse heart, Rat heart; Cellular Location: Nucleus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.63 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HOXD10 in mouse kidney. Samples were incubated with HOXD10 Polyclonal antibody (Product # PA5-96759) using a dilution of 1:800, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HOXD10 was performed in U2OS cells using HOXD10 Polyclonal Antibody (Product # PA5-96759) at a dilution of 1:100. Blue: DAPI for nuclear staining.