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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00003283-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003283-D01, RRID:AB_10720600
- Product name
- HSD3B1 MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human HSD3B1 protein.
- Antigen sequence
MTGWSCLVTGAGGFLGQRIIRLLVKEKELKEIRVL
DKAFGPELREEFSKLQNKTKLTVLEGDILDEPFLK
RACQDVSVIIHTACIIDVFGVTHRESIMNVNVKGT
QLLLEACVQASVPVFIYTSSIEVAGPNSYKEIIQN
GHEEEPLENTWPAPYPHSKKLAEKAVLAANGWNLK
NGGTLYTCALRPMYIYGEGSRFLSASINEALNNNG
ILSSVGKFSTVNPVYVGNVAWAHILALRALQDPKK
APSIRGQFYYISDDTPHQSYDNLNYTLSKEFGLRL
DSRWSFPLSLMYWIGFLLEIVSFLLRPIYTYRPPF
NRHIVTLSNSVFTFSYKKAQRDLAYKPLYSWEEAK
QKTVEWVGSLVDRHKETLKSKTQ- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of HSD3B1 expression in transfected 293T cell line (H00003283-T01) by HSD3B1 MaxPab polyclonal antibody.Lane 1: HSD3B1 transfected lysate(42.3 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HSD3B1 MaxPab rabbit polyclonal antibody. Western Blot analysis of HSD3B1 expression in MCF-7.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of HSD3B1 transfected lysate using anti-HSD3B1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HSD3B1 purified MaxPab mouse polyclonal antibody (B01P) (H00003283-B01P).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
