Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-18503 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HSD3B1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Placenta lysate using Product # PA5-18503 at a concentration of 0.01 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Placenta lysate using Product # PA5-18503 at a concentration of 0.01 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of HEK293 overexpressing Human HSD3B1 using Product # PA5-18503, mock transfection as a control in first lane.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HSD3B1 by a HSD3B1 monoclonal antibody (Product # PA5-18503) at a concentration of 0.003 µg/mL. Human Adrenal Gland lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HSD3B1 in Human Placenta using a HSD3B1 monoclonal antibody (Product #PA5-18503) at 3.75 µg/mL. The Human Placenta tissue section was paraffin embeded and detected using steamed antigen retrieval with citrate buffer pH 6, AP-staining.