PA1-28532

antibody from Invitrogen Antibodies

Targeting: COL3A1 EDS4A

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-28532

Provider

Invitrogen Antibodies

Product name

Collagen III Polyclonal Antibody, Biotin

Antibody type

Polyclonal

Antigen

Other

Description

PA1-28532 detects Collagen III from bovine, human samples.

Reactivity

Human, Mouse, Rat, Bovine

Host

Rabbit

Conjugate

Biotin

Isotype

IgG

Vial size

50 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Collagen III was performed in human skin and testis tissue using Collagen III Polyclonal Antibody, Biotin (Product # PA1-28532) at a dilution of 1:400 for 45 minutes a room temperature. The antibody showed strong staining in FFPE sections of human skin (left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.

Conjugate

Biotin