PA1-28870

antibody from Invitrogen Antibodies

Targeting: COL3A1 EDS4A

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-28870

Provider

Invitrogen Antibodies

Product name

Collagen III Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

PA1-28870 has been successfully used in ELISA, immunohistochemistry (frozen tissue), immunoprecipitation, and Western blot applications. NOTE: The preparation of a specific antibody to Collagen III's uninterrupted "Glycine-X-Y" triplet repeat requires a non-denatured epitope format. Consequently, it is recommended to use this antibody in a Western blot using non-denaturing or "native" conditions.

Concentration

1.16 mg/mL

References

Submitted references

Two-photon fluorescence and second harmonic generation characterization of extracellular matrix remodeling in post-injury murine temporomandibular joint osteoarthritis.

Reed DA, Yotsuya M, Gubareva P, Toth PT, Bertagna A
PloS one 2019;14(3):e0214072

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Collagen III was performed by loading 100 ng of Human Collagen III protein. Primary antibody: Collagen III Polyclonal Antibody (Product # PA1-28870) at a dilution of 1:1000 overnight at 4°C. Secondary antibody: Goat anti-rabbit at 1:20,000 for 30 min at RT Block for 30 min at RT. Predicted/Observed size: 138 kDa, 138 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Collagen III was performed in human skin and testis tissue using Collagen III Polyclonal Antibody (Product # PA1-28870) at a dilution of 1:400 for 45 minutes a room temperature. The antibody showed strong staining in FFPE sections of human skin (left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

10.1371/journal.pone.0214072.g005 Fig 5 Changes in superficial layer collagens during early stage TMJ OA. Immunolabeling of fibrillar collagens in the TMJ illustrating changes in the composition of the superficial layer extracellular matrix that accompanies the progression of early stage TMJ-OA. Collagen I (A-C) is immunolabeled using a brown chromogen. Collagen II (D-F) and Collagen III (G-I) are immunolabeled using a pink chromogen. Cell nuclei are labeled in purple with hematoxylin. Tissues from non-surgical controls (A, D, G) are compared with 4 (B, E, H) and 8 (C, F, I) week post-injury tissues. All images are representative of their experimental group (n = 3) and taken from homologous regions of the mandibular condyle. Differences in the shape of the condylar reflect variable amounts of condylar flattening, typical of the surgical model. Medial is to the right in all joints. Scale bars equal 50 mum. Note strong staining for Collagens I/III in the superficial layer in post-injury tissues, with Collagen II restricted to the deeper chondroblastic/hypertrophic layers. Primary and secondary antibody controls were negative.