Antibody data
- Antibody Data
- Antigen structure
- References [39]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- A00788-3 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Collagen III/COL3A1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Collagen III/COL3A1 detection. Tested with WB, IHC-P, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C; for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Endogenous hydrogen sulphide deficiency and exogenous hydrogen sulphide supplement regulate skin fibroblasts proliferation via necroptosis.
Impaired pulp healing associated with underlying disorders in the dental pulp of rats with type 2 diabetes.
Epigallocatechin-3-Gallate Inhibits Atrial Fibrosis and Reduces the Occurrence and Maintenance of Atrial Fibrillation and its Possible Mechanisms.
Timosaponin BII inhibits TGF-β mediated epithelial-mesenchymal transition through Smad-dependent pathway during pulmonary fibrosis.
Patchouli alcohol against renal fibrosis of spontaneously hypertensive rats via Ras/Raf-1/ERK1/2 signalling pathway.
Salvianolic acid B ameliorates myocardial fibrosis in diabetic cardiomyopathy by deubiquitinating Smad7.
Hydrogen Sulfide Suppresses Skin Fibroblast Proliferation via Oxidative Stress Alleviation and Necroptosis Inhibition.
Pirfenidone Attenuates Renal Tubulointerstitial Fibrosis through Inhibiting miR-21.
Platelet-rich plasma pretreatment protects anterior cruciate ligament fibroblasts correlated with PI3K-Akt-mTOR pathway under hypoxia condition.
Human ucMSCs seeded in a decellularized kidney scaffold attenuate renal fibrosis by reducing epithelial-mesenchymal transition via the TGF-β/Smad signaling pathway.
The protective role of Schwann cells in bladder smooth muscle cell fibrosis.
Piperine ameliorates collagenase-induced Achilles tendon injury in the rat.
Effect of genistein on myocardial fibrosis in diabetic rats and its mechanism.
YKL-40 is highly expressed in the epicardial adipose tissue of patients with atrial fibrillation and associated with atrial fibrosis.
Long-term intravenous administration of carboxylated single-walled carbon nanotubes induces persistent accumulation in the lungs and pulmonary fibrosis via the nuclear factor-kappa B pathway.
Deficiency of mPGES-1 exacerbates renal fibrosis and inflammation in mice with unilateral ureteral obstruction.
Protective role of ACE2-Ang-(1-7)-Mas in myocardial fibrosis by downregulating K(Ca)3.1 channel via ERK1/2 pathway.
miR-410 suppresses the expression of interleukin-6 as well as renal fibrosis in the pathogenesis of lupus nephritis.
Hypoxia enhances the protective effects of placenta-derived mesenchymal stem cells against scar formation through hypoxia-inducible factor-1α.
Skin healing and collagen changes of rats after fractional erbium:yttrium aluminum garnet laser: observation by reflectance confocal microscopy with confirmed histological evidence.
CYP epoxygenase 2J2 prevents cardiac fibrosis by suppression of transmission of pro-inflammation from cardiomyocytes to macrophages.
Mechanisms of nerve capping technique in prevention of painful neuroma formation.
Rapamycin slows IgA nephropathy progression in the rat.
β-catenin is overexpressed in hepatic fibrosis and blockage of Wnt/β-catenin signaling inhibits hepatic stellate cell activation.
Localization of human mesenchymal stem cells from umbilical cord blood and their role in repair of diabetic foot ulcers in rats.
Anti-fibrotic effects of puerarin on CCl4-induced hepatic fibrosis in rats possibly through the regulation of PPAR-γ expression and inhibition of PI3K/Akt pathway.
Thrombospondin-1 short hairpin RNA suppresses tubulointerstitial fibrosis in the kidney of ureteral obstruction by ameliorating peritubular capillary injury.
Inhibition of high-mobility group box 1 expression by siRNA in rat hepatic stellate cells.
The effect of down-regulation of Smad3 by RNAi on hepatic stellate cells and a carbon tetrachloride-induced rat model of hepatic fibrosis.
The role of mast cells in non-ablative laser resurfacing with 1,320 nm neodymium:yttrium-aluminium-garnet laser.
Effects of exogenous urotensin II on vascular remodelling after balloon injury.
Attenuation of portal hypertension by natural taurine in rats with liver cirrhosis.
Fluvastatin decreases cardiac fibrosis possibly through regulation of TGF-beta(1)/Smad 7 expression in the spontaneously hypertensive rats.
Inhibition of plasminogen activator inhibitor-1 expression by siRNA in rat hepatic stellate cells.
Modified synthetic siRNA targeting tissue inhibitor of metalloproteinase-2 inhibits hepatic fibrogenesis in rats.
Isolation of neural crest-derived stem cells from rat embryonic mandibular processes.
Treatment of experimental hepatic fibrosis by combinational delivery of urokinase-type plasminogen activator and hepatocyte growth factor genes.
Role of impaired peritubular capillary and hypoxia in progressive interstitial fibrosis after 56 subtotal nephrectomy of rats.
Effects of pentoxifylline on the hepatic content of TGF-beta1 and collagen in Schistosomiasis japonica mice with liver fibrosis.
Li L, Chen X, Liu C, He Z, Shen Q, Zhu Y, Wang X, Cao S, Yang S
Experimental dermatology 2024 Jan;33(1):e14972
Experimental dermatology 2024 Jan;33(1):e14972
Impaired pulp healing associated with underlying disorders in the dental pulp of rats with type 2 diabetes.
Tao S, Yang T, Zhou JN, Zhang Q
Journal of dental sciences 2024 Jan;19(1):310-320
Journal of dental sciences 2024 Jan;19(1):310-320
Epigallocatechin-3-Gallate Inhibits Atrial Fibrosis and Reduces the Occurrence and Maintenance of Atrial Fibrillation and its Possible Mechanisms.
Li T, Tong Q, Wang Z, Yang Z, Sun Y, Cai J, Xu Q, Lu Y, Liu X, Lin K, Qian Y
Cardiovascular drugs and therapy 2023 Mar 31;
Cardiovascular drugs and therapy 2023 Mar 31;
Timosaponin BII inhibits TGF-β mediated epithelial-mesenchymal transition through Smad-dependent pathway during pulmonary fibrosis.
Ding D, Shen X, Yu L, Zheng Y, Liu Y, Wang W, Liu L, Zhao Z, Nian S, Liu L
Phytotherapy research : PTR 2023 Jul;37(7):2787-2799
Phytotherapy research : PTR 2023 Jul;37(7):2787-2799
Patchouli alcohol against renal fibrosis of spontaneously hypertensive rats via Ras/Raf-1/ERK1/2 signalling pathway.
Li J, Hu G, Liu W, Cao X, Chen G, Peng F, Xiaofang X, Peng C
The Journal of pharmacy and pharmacology 2023 Jul 5;75(7):995-1010
The Journal of pharmacy and pharmacology 2023 Jul 5;75(7):995-1010
Salvianolic acid B ameliorates myocardial fibrosis in diabetic cardiomyopathy by deubiquitinating Smad7.
Luo H, Fu L, Wang X, Yini Xu, Ling Tao, Shen X
Chinese medicine 2023 Dec 10;18(1):161
Chinese medicine 2023 Dec 10;18(1):161
Hydrogen Sulfide Suppresses Skin Fibroblast Proliferation via Oxidative Stress Alleviation and Necroptosis Inhibition.
Li L, He Z, Zhu Y, Shen Q, Yang S, Cao S
Oxidative medicine and cellular longevity 2022;2022:7434733
Oxidative medicine and cellular longevity 2022;2022:7434733
Pirfenidone Attenuates Renal Tubulointerstitial Fibrosis through Inhibiting miR-21.
Bi L, Huang Y, Li J, Yang X, Hou G, Zhai P, Zhang Q, Alhaji AA, Yang Y, Liu B
Nephron 2022;146(1):110-120
Nephron 2022;146(1):110-120
Platelet-rich plasma pretreatment protects anterior cruciate ligament fibroblasts correlated with PI3K-Akt-mTOR pathway under hypoxia condition.
Cao Y, Li Y, Fu SC, Shen J, Zhang H, Jiang C, Shu-Hang Yung P
Journal of orthopaedic translation 2022 May;34:102-112
Journal of orthopaedic translation 2022 May;34:102-112
Human ucMSCs seeded in a decellularized kidney scaffold attenuate renal fibrosis by reducing epithelial-mesenchymal transition via the TGF-β/Smad signaling pathway.
Hu D, Zhang D, Liu B, Liu Y, Zhou Y, Yu Y, Shen L, Long C, Zhang D, Liu X, Lin T, He D, Xu T, Timashev P, Butnaru D, Zhang Y, Wei G
Pediatric research 2020 Aug;88(2):192-201
Pediatric research 2020 Aug;88(2):192-201
The protective role of Schwann cells in bladder smooth muscle cell fibrosis.
Liu Q, Wang R, Wang C, Li Y, Li A
International journal of clinical and experimental pathology 2019;12(10):3799-3806
International journal of clinical and experimental pathology 2019;12(10):3799-3806
Piperine ameliorates collagenase-induced Achilles tendon injury in the rat.
Gong F, Cui L, Zhang X, Zhan X, Gong X, Wen Y
Connective tissue research 2018 Jan;59(1):21-29
Connective tissue research 2018 Jan;59(1):21-29
Effect of genistein on myocardial fibrosis in diabetic rats and its mechanism.
Yang R, Jia Q, Liu XF, Ma SF
Molecular medicine reports 2018 Feb;17(2):2929-2936
Molecular medicine reports 2018 Feb;17(2):2929-2936
YKL-40 is highly expressed in the epicardial adipose tissue of patients with atrial fibrillation and associated with atrial fibrosis.
Wang Q, Shen H, Min J, Gao Y, Liu K, Xi W, Yang J, Yin L, Xu J, Xiao J, Wang Z
Journal of translational medicine 2018 Aug 15;16(1):229
Journal of translational medicine 2018 Aug 15;16(1):229
Long-term intravenous administration of carboxylated single-walled carbon nanotubes induces persistent accumulation in the lungs and pulmonary fibrosis via the nuclear factor-kappa B pathway.
Qin Y, Li S, Zhao G, Fu X, Xie X, Huang Y, Cheng X, Wei J, Liu H, Lai Z
International journal of nanomedicine 2017;12:263-277
International journal of nanomedicine 2017;12:263-277
Deficiency of mPGES-1 exacerbates renal fibrosis and inflammation in mice with unilateral ureteral obstruction.
Luo R, Kakizoe Y, Wang F, Fan X, Hu S, Yang T, Wang W, Li C
American journal of physiology. Renal physiology 2017 Jan 1;312(1):F121-F133
American journal of physiology. Renal physiology 2017 Jan 1;312(1):F121-F133
Protective role of ACE2-Ang-(1-7)-Mas in myocardial fibrosis by downregulating K(Ca)3.1 channel via ERK1/2 pathway.
Wang LP, Fan SJ, Li SM, Wang XJ, Gao JL, Yang XH
Pflugers Archiv : European journal of physiology 2016 Nov;468(11-12):2041-2051
Pflugers Archiv : European journal of physiology 2016 Nov;468(11-12):2041-2051
miR-410 suppresses the expression of interleukin-6 as well as renal fibrosis in the pathogenesis of lupus nephritis.
Liu D, Zhang N, Zhang J, Zhao H, Wang X
Clinical and experimental pharmacology & physiology 2016 Jun;43(6):616-25
Clinical and experimental pharmacology & physiology 2016 Jun;43(6):616-25
Hypoxia enhances the protective effects of placenta-derived mesenchymal stem cells against scar formation through hypoxia-inducible factor-1α.
Du L, Lv R, Yang X, Cheng S, Xu J, Ma T
Biotechnology letters 2016 Jun;38(6):931-9
Biotechnology letters 2016 Jun;38(6):931-9
Skin healing and collagen changes of rats after fractional erbium:yttrium aluminum garnet laser: observation by reflectance confocal microscopy with confirmed histological evidence.
Yang J, Wang S, Dong L, An X, Li Y, Li J, Tu Y, Tao J
Lasers in medical science 2016 Aug;31(6):1251-60
Lasers in medical science 2016 Aug;31(6):1251-60
CYP epoxygenase 2J2 prevents cardiac fibrosis by suppression of transmission of pro-inflammation from cardiomyocytes to macrophages.
Yang L, Ni L, Duan Q, Wang X, Chen C, Chen S, Chaugai S, Zeldin DC, Tang JR, Wang DW
Prostaglandins & other lipid mediators 2015 Jan-Mar;116-117:64-75
Prostaglandins & other lipid mediators 2015 Jan-Mar;116-117:64-75
Mechanisms of nerve capping technique in prevention of painful neuroma formation.
Yan H, Zhang F, Kolkin J, Wang C, Xia Z, Fan C
PloS one 2014;9(4):e93973
PloS one 2014;9(4):e93973
Rapamycin slows IgA nephropathy progression in the rat.
Tian J, Wang Y, Zhou X, Li Y, Wang C, Li J, Li R
American journal of nephrology 2014;39(3):218-29
American journal of nephrology 2014;39(3):218-29
β-catenin is overexpressed in hepatic fibrosis and blockage of Wnt/β-catenin signaling inhibits hepatic stellate cell activation.
Ge WS, Wang YJ, Wu JX, Fan JG, Chen YW, Zhu L
Molecular medicine reports 2014 Jun;9(6):2145-51
Molecular medicine reports 2014 Jun;9(6):2145-51
Localization of human mesenchymal stem cells from umbilical cord blood and their role in repair of diabetic foot ulcers in rats.
Zhao QS, Xia N, Zhao N, Li M, Bi CL, Zhu Q, Qiao GF, Cheng ZF
International journal of biological sciences 2013;10(1):80-9
International journal of biological sciences 2013;10(1):80-9
Anti-fibrotic effects of puerarin on CCl4-induced hepatic fibrosis in rats possibly through the regulation of PPAR-γ expression and inhibition of PI3K/Akt pathway.
Guo C, Xu L, He Q, Liang T, Duan X, Li R
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2013 Jun;56:436-42
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2013 Jun;56:436-42
Thrombospondin-1 short hairpin RNA suppresses tubulointerstitial fibrosis in the kidney of ureteral obstruction by ameliorating peritubular capillary injury.
Sun D, Ma Y, Han H, Yin Z, Liu C, Feng J, Zhou X, Li X, Xiao A, Yu R
Kidney & blood pressure research 2012;35(1):35-47
Kidney & blood pressure research 2012;35(1):35-47
Inhibition of high-mobility group box 1 expression by siRNA in rat hepatic stellate cells.
Ge WS, Wu JX, Fan JG, Wang YJ, Chen YW
World journal of gastroenterology 2011 Sep 28;17(36):4090-8
World journal of gastroenterology 2011 Sep 28;17(36):4090-8
The effect of down-regulation of Smad3 by RNAi on hepatic stellate cells and a carbon tetrachloride-induced rat model of hepatic fibrosis.
Wang ZR, Wang JH, Hu CL, Cao WG, Shen XJ, Wu MY, Shen L, Wu SL
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2011 Feb;44(2):91-9
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2011 Feb;44(2):91-9
The role of mast cells in non-ablative laser resurfacing with 1,320 nm neodymium:yttrium-aluminium-garnet laser.
Shang Y, Wang Z, Pang Y, Xi P, Ren Q
Lasers in medical science 2010 May;25(3):371-7
Lasers in medical science 2010 May;25(3):371-7
Effects of exogenous urotensin II on vascular remodelling after balloon injury.
Zhang LF, Ding WH, Shi LB, Li K, Haom YJ, Ke YN, Tang ZS
Clinical and experimental pharmacology & physiology 2010 Apr;37(4):477-81
Clinical and experimental pharmacology & physiology 2010 Apr;37(4):477-81
Attenuation of portal hypertension by natural taurine in rats with liver cirrhosis.
Liang J, Deng X, Lin ZX, Zhao LC, Zhang XL
World journal of gastroenterology 2009 Sep 28;15(36):4529-37
World journal of gastroenterology 2009 Sep 28;15(36):4529-37
Fluvastatin decreases cardiac fibrosis possibly through regulation of TGF-beta(1)/Smad 7 expression in the spontaneously hypertensive rats.
Zhai Y, Gao X, Wu Q, Peng L, Lin J, Zuo Z
European journal of pharmacology 2008 Jun 10;587(1-3):196-203
European journal of pharmacology 2008 Jun 10;587(1-3):196-203
Inhibition of plasminogen activator inhibitor-1 expression by siRNA in rat hepatic stellate cells.
Hu PF, Zhu YW, Zhong W, Chen YX, Lin Y, Zhang X, Yin C, Yue HY, Xie WF
Journal of gastroenterology and hepatology 2008 Dec;23(12):1917-25
Journal of gastroenterology and hepatology 2008 Dec;23(12):1917-25
Modified synthetic siRNA targeting tissue inhibitor of metalloproteinase-2 inhibits hepatic fibrogenesis in rats.
Hu YB, Li DG, Lu HM
The journal of gene medicine 2007 Mar;9(3):217-29
The journal of gene medicine 2007 Mar;9(3):217-29
Isolation of neural crest-derived stem cells from rat embryonic mandibular processes.
Zhang J, Duan X, Zhang H, Deng Z, Zhou Z, Wen N, Smith AJ, Zhao W, Jin Y
Biology of the cell 2006 Oct;98(10):567-75
Biology of the cell 2006 Oct;98(10):567-75
Treatment of experimental hepatic fibrosis by combinational delivery of urokinase-type plasminogen activator and hepatocyte growth factor genes.
Lin Y, Xie WF, Chen YX, Zhang X, Zeng X, Qiang H, Chen WZ, Yang XJ, Han ZG, Zhang ZB
Liver international : official journal of the International Association for the Study of the Liver 2005 Aug;25(4):796-807
Liver international : official journal of the International Association for the Study of the Liver 2005 Aug;25(4):796-807
Role of impaired peritubular capillary and hypoxia in progressive interstitial fibrosis after 56 subtotal nephrectomy of rats.
Zhang B, Liang X, Shi W, Ye Z, He C, Hu X, Liu S
Nephrology (Carlton, Vic.) 2005 Aug;10(4):351-7
Nephrology (Carlton, Vic.) 2005 Aug;10(4):351-7
Effects of pentoxifylline on the hepatic content of TGF-beta1 and collagen in Schistosomiasis japonica mice with liver fibrosis.
Xiong LJ, Zhu JF, Luo DD, Zen LL, Cai SQ
World journal of gastroenterology 2003 Jan;9(1):152-4
World journal of gastroenterology 2003 Jan;9(1):152-4
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of Collagen III using anti-Collagen III antibody (A00788-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysate,Lane 2: human U2OS whole cell lysate,Lane 3: human SW620 whole cell lysate,Lane 4: human SGC-7901 whole cell lysate,Lane 5: rat heart tissue lysate,Lane 6: rat skeletal muscle tissue lysate,Lane 7: mouse skeletal muscle tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen III antigen affinity purified polyclonal antibody (Catalog # A00788-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen III at approximately 160KD. The expected band size for Collagen III is at 139KD.
- Additional image