NBP1-26547

antibody from Novus Biologicals

Targeting: COL3A1 EDS4A

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-26547

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-26547, RRID:AB_1853195

Product name

Goat Polyclonal Collagen III alpha 1/COL3A1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Reacts with conformational determinants on human type III collagen as demonstrated by ELISA. May react with type III collagen from other species. Exhibits

Reactivity

Human, Mouse, Rat, Bovine, Canine

Host

Goat

Isotype

IgG

Vial size

0.1 mg

Concentration

0.4 mg/ml

Storage

Store at 4C. Do not freeze.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NBP1-26547] - Human gastric cancer tissue was stained with Goat Anti-Type III Collagen-UNLB followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NBP1-26547] - Paraplast Plus (R) embedded 2K1C rat kidney section was stained with Goat Anti-Type III Collagen-UNLB followed by a secondary antibody and DAB and hematoxylin. Image from Cuevas CA, Tapia-Rojas C, Cespedes C, Inestrosa NC, Vio CP. beta-catenin-dependent signaling pathway contributes to renal fibrosis in hypertensive rats. Biomed Res Int. 2015;2015:726012.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: Collagen III alpha 1/COL3A1 Antibody [NBP1-26547] - Frozen osteopontin-null mouse muscle section was stained with Goat Anti-Type III Collagen-UNLB followed by a secondary antibody. Image from Gawlik KI, Holmberg J, Svensson M, Einerborg M, Oliveira BM, Deierborg T. Potent pro-inflammatory and pro-fibrotic molecules, osteopontin and galectin-3, are not major disease modulators of laminin alpha2 chain-deficient muscular dystrophy. Sci Rep. 2017;10:1038.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: Collagen III alpha 1/COL3A1 Antibody [NBP1-26547] - Frozen kidney section following unilateral ureteral obstruction was stained with Goat Anti-Type III Collagen-UNLB followed by a secondary antibody. Image from Maeshima A, Mishima K, Yamashita S, Nakasatomi M, Miya M, Sakurai T, et al. Follistatin, an activin antagonist, ameliorates renal interstitial fibrosis in a rat model of unilateral ureteral obstruction. Biomed Res Int. 2014;2014:376191.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NBP1-26547] - Paraffin embedded doxorubicin treated rat kidney section was stained with Goat Anti-Type III Collagen-UNLB followed by a secondary antibody and DAB. Image from Hijmans RS, Rasmussen DG, Yazdani S, Navis G, van Goor H, Karsdal MA, et al. Urinary collagen degradation products as early markers of progressive renal fibrosis. J Transl Med. 2017;15:63.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NBP1-26547] - Human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (left) and Goat Anti-Type III Collagen-UNLB (right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP DAB, and hematoxylin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NBP1-26547] - Human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (left) and Goat Anti-Type III Collagen-UNLB (right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP DAB, and hematoxy