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Western blot
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- Antibody Data
- Antigen structure
- References [15]
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- Validations
- Western blot [1]
- Immunohistochemistry [4]
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- Product number
- NB600-594 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-594, RRID:AB_10001330
- Product name
- Rabbit Polyclonal Collagen III alpha 1/COL3A1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Typically less than 1% cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type III collagens and has negligible cross-reactivity with Type I, II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Submitted references Fast, robust and effective decellularization of whole human livers using mild detergents and pressure controlled perfusion.
Radiation cystitis modeling: A comparative study of bladder fibrosis radio-sensitivity in C57BL/6, C3H, and BALB/c mice.
Salvia miltiorrhiza and Carthamus tinctorius Extract Prevents Cardiac Fibrosis and Dysfunction after Myocardial Infarction by Epigenetically Inhibiting Smad3 Expression.
AIM2 gene silencing attenuates diabetic cardiomyopathy in type 2 diabetic rat model.
Inhibition of the Akt1-mTORC1 Axis Alters Venous Remodeling to Improve Arteriovenous Fistula Patency.
Bilateral sympathetic stellate ganglionectomy attenuates myocardial remodelling and fibrosis in a rat model of chronic volume overload.
Mapping Extracellular Matrix Proteins in Formalin-Fixed, Paraffin-Embedded Tissues by MALDI Imaging Mass Spectrometry.
Effects of 6-months' Exercise on Cardiac Function, Structure and Metabolism in Female Hypertensive Rats-The Decisive Role of Lysyl Oxidase and Collagen III.
Angiopoietin-like 4 induces a β-catenin-mediated upregulation of ID3 in fibroblasts to reduce scar collagen expression.
Effect of a single subacromial prednisolone injection in acute rotator cuff tears in a rat model.
Liver fibrosis in elderly cadavers: localization of collagen types I, III, and IV, α-smooth muscle actin, and elastic fibers.
Hepatic steatosis, fibrosis, and cancer in elderly cadavers.
Comparison of two murine models of thrombosis induced by atherosclerotic plaque injury.
Readily available tissue-engineered vascular grafts.
Protein precoating of elastomeric tissue-engineering scaffolds increased cellularity, enhanced extracellular matrix protein production, and differentially regulated the phenotypes of circulating endothelial progenitor cells.
Willemse J, Verstegen MMA, Vermeulen A, Schurink IJ, Roest HP, van der Laan LJW, de Jonge J
Materials science & engineering. C, Materials for biological applications 2020 Mar;108:110200
Materials science & engineering. C, Materials for biological applications 2020 Mar;108:110200
Radiation cystitis modeling: A comparative study of bladder fibrosis radio-sensitivity in C57BL/6, C3H, and BALB/c mice.
Zwaans BMM, Wegner KA, Bartolone SN, Vezina CM, Chancellor MB, Lamb LE
Physiological reports 2020 Feb;8(4):e14377
Physiological reports 2020 Feb;8(4):e14377
Salvia miltiorrhiza and Carthamus tinctorius Extract Prevents Cardiac Fibrosis and Dysfunction after Myocardial Infarction by Epigenetically Inhibiting Smad3 Expression.
Yang J, Wang B, Li N, Zhou Q, Zhou W, Zhan Z
Evidence-based complementary and alternative medicine : eCAM 2019;2019:6479136
Evidence-based complementary and alternative medicine : eCAM 2019;2019:6479136
AIM2 gene silencing attenuates diabetic cardiomyopathy in type 2 diabetic rat model.
Wang X, Pan J, Liu H, Zhang M, Liu D, Lu L, Tian J, Liu M, Jin T, An F
Life sciences 2019 Mar 15;221:249-258
Life sciences 2019 Mar 15;221:249-258
Inhibition of the Akt1-mTORC1 Axis Alters Venous Remodeling to Improve Arteriovenous Fistula Patency.
Guo X, Fereydooni A, Isaji T, Gorecka J, Liu S, Hu H, Ono S, Alozie M, Lee SR, Taniguchi R, Yatsula B, Nassiri N, Zhang L, Dardik A
Scientific reports 2019 Jul 30;9(1):11046
Scientific reports 2019 Jul 30;9(1):11046
Bilateral sympathetic stellate ganglionectomy attenuates myocardial remodelling and fibrosis in a rat model of chronic volume overload.
Zhang M, Zhu P, Wang Y, Wu J, Yu Y, Wu X, Liu X, Gu Y
Journal of cellular and molecular medicine 2019 Feb;23(2):1001-1013
Journal of cellular and molecular medicine 2019 Feb;23(2):1001-1013
Mapping Extracellular Matrix Proteins in Formalin-Fixed, Paraffin-Embedded Tissues by MALDI Imaging Mass Spectrometry.
Angel PM, Comte-Walters S, Ball LE, Talbot K, Mehta A, Brockbank KGM, Drake RR
Journal of proteome research 2018 Jan 5;17(1):635-646
Journal of proteome research 2018 Jan 5;17(1):635-646
Effects of 6-months' Exercise on Cardiac Function, Structure and Metabolism in Female Hypertensive Rats-The Decisive Role of Lysyl Oxidase and Collagen III.
Schreckenberg R, Horn AM, da Costa Rebelo RM, Simsekyilmaz S, Niemann B, Li L, Rohrbach S, Schlüter KD
Frontiers in physiology 2017;8:556
Frontiers in physiology 2017;8:556
Angiopoietin-like 4 induces a β-catenin-mediated upregulation of ID3 in fibroblasts to reduce scar collagen expression.
Teo Z, Chan JSK, Chong HC, Sng MK, Choo CC, Phua GZM, Teo DJR, Zhu P, Choong C, Wong MTC, Tan NS
Scientific reports 2017 Jul 24;7(1):6303
Scientific reports 2017 Jul 24;7(1):6303
Effect of a single subacromial prednisolone injection in acute rotator cuff tears in a rat model.
Lee HJ, Kim YS, Ok JH, Lee YK, Ha MY
Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA 2015 Feb;23(2):555-61
Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA 2015 Feb;23(2):555-61
Liver fibrosis in elderly cadavers: localization of collagen types I, III, and IV, α-smooth muscle actin, and elastic fibers.
Mak KM, Chu E, Lau KH, Kwong AJ
Anatomical record (Hoboken, N.J. : 2007) 2012 Jul;295(7):1159-67
Anatomical record (Hoboken, N.J. : 2007) 2012 Jul;295(7):1159-67
Hepatic steatosis, fibrosis, and cancer in elderly cadavers.
Mak KM, Kwong AJ, Chu E, Hoo NM
Anatomical record (Hoboken, N.J. : 2007) 2012 Jan;295(1):40-50
Anatomical record (Hoboken, N.J. : 2007) 2012 Jan;295(1):40-50
Comparison of two murine models of thrombosis induced by atherosclerotic plaque injury.
Hechler B, Gachet C
Thrombosis and haemostasis 2011 May;105 Suppl 1:S3-12
Thrombosis and haemostasis 2011 May;105 Suppl 1:S3-12
Readily available tissue-engineered vascular grafts.
Dahl SL, Kypson AP, Lawson JH, Blum JL, Strader JT, Li Y, Manson RJ, Tente WE, DiBernardo L, Hensley MT, Carter R, Williams TP, Prichard HL, Dey MS, Begelman KG, Niklason LE
Science translational medicine 2011 Feb 2;3(68):68ra9
Science translational medicine 2011 Feb 2;3(68):68ra9
Protein precoating of elastomeric tissue-engineering scaffolds increased cellularity, enhanced extracellular matrix protein production, and differentially regulated the phenotypes of circulating endothelial progenitor cells.
Sales VL, Engelmayr GC Jr, Johnson JA Jr, Gao J, Wang Y, Sacks MS, Mayer JE Jr
Circulation 2007 Sep 11;116(11 Suppl):I55-63
Circulation 2007 Sep 11;116(11 Suppl):I55-63
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Lane 1: Human Collagen III Load: 100 ng per lane Primary antibody: Collagen III Antibody at 1:1000 o/n at 4C Secondary antibody: DyLight 649 Goat anti-rabbit at 1:20,000 for 30 min at RT Block: incubated with blocking buffer for 30 min at RT Predicted/Observed size: 138 kDa, 138 kDa
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Tissue: right lobe of the liver section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow). Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-collagen type I at 1:500 for 4 degrees Celsius for 24hr. Secondary antibody: Peroxidase biotin-streptavidin rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Anti-collagen type III is intra and extracellular. Staining: 3.3'-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Human lung tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Human lung tissue
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Analysis of FITC conjugate of NB600-594. Staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody s
