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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- R1040 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1040, RRID:AB_978547
- Product name
- anti Collagen type III alpha 1 chain
- Antibody type
- Polyclonal
- Antigen
- Collagen type III purified from Human and Bovine placenta
- Reactivity
- Human, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot of rabbit collagen III alpha 1 chain antibody Cat.-No. R1040, Lane 1: human collagen III load: 100 ng per lane, primary antibody: Collagen III antibody at 1:1000 o/n at 4°C, secondary antibody: DyLight⢠649 goat anti-rabbit.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry of tissue of right lobe of the liver section (Formalin-fixed, Paraffin-embedded). A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow) using Collagen type III alpha 1 chain antibody Cat.-No. R1040 at 1:500 for 4°C for 24hr. Secondary antibody: Peroxidase biotin-streptavidin rabbit secondary antibody and DAB as chromogen. Nuclei were counterstained purple with hematoxylin.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- R1040 Collagen III Antibody (Lot 26016, 1/400, 45 min RT) showed strong staining in FFPE sections of Human skin (Left, dermis) with moderate to strong Red staining and testis (Right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA
