21393-1-AP

antibody from Proteintech Group

Targeting: RNF168 FLJ35794

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

21393-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#21393-1-AP, RRID:AB_10733883

Product name

RNF168 antibody

Antibody type

Polyclonal

Description

RNF168 antibody (Cat. #21393-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IP, WB, ELISA.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis.

Bao K, Ma Y, Li Y, Shen X, Zhao J, Tian S, Zhang C, Liang C, Zhao Z, Yang Y, Zhang K, Yang N, Meng FL, Hao J, Yang J, Liu T, Yao Z, Ai D, Shi L
Molecular cell 2024 Apr 4;84(7):1206-1223.e15

---

The HDAC6-RNF168 axis regulates H2A/H2A.X ubiquitination to enable double-strand break repair.

Qiu L, Xu W, Lu X, Chen F, Chen Y, Tian Y, Zhu Q, Liu X, Wang Y, Pei XH, Xu X, Zhang J, Zhu WG
Nucleic acids research 2023 Sep 22;51(17):9166-9182

---

SENP1 Decreases RNF168 Phase Separation to Promote DNA Damage Repair and Drug Resistance in Colon Cancer.

Wei M, Huang X, Liao L, Tian Y, Zheng X
Cancer research 2023 Sep 1;83(17):2908-2923

---

A 1,2,3-Triazole Derivative of Quinazoline Exhibits Antitumor Activity by Tethering RNF168 to SQSTM1/P62.

Wang FC, Peng B, Ren TT, Liu SP, Du JR, Chen ZH, Zhang TT, Gu X, Li M, Cao SL, Xu X
Journal of medicinal chemistry 2022 Nov 24;65(22):15028-15047

---

Non-canonical function of DGCR8 in DNA double-strand break repair signaling and tumor radioresistance.

Hang Q, Zeng L, Wang L, Nie L, Yao F, Teng H, Deng Y, Yap S, Sun Y, Frank SJ, Chen J, Ma L
Nature communications 2021 Jun 29;12(1):4033

---

RNF8-ubiquitinated KMT5A is required for RNF168-induced H2A ubiquitination in response to DNA damage.

Lu X, Xu M, Zhu Q, Zhang J, Liu G, Bao Y, Gu L, Tian Y, Wen H, Zhu WG
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Apr;35(4):e21326

---

A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis.

Zhang Q, Cao C, Gong W, Bao K, Wang Q, Wang Y, Bi L, Ma S, Zhao J, Liu L, Tian S, Zhang K, Yang J, Yao Z, Song N, Shi L
Theranostics 2020;10(23):10769-10790

---

An LTR retrotransposon-derived lncRNA interacts with RNF169 to promote homologous recombination.

Deng B, Xu W, Wang Z, Liu C, Lin P, Li B, Huang Q, Yang J, Zhou H, Qu L
EMBO reports 2019 Nov 5;20(11):e47650

---

GLP-catalyzed H4K16me1 promotes 53BP1 recruitment to permit DNA damage repair and cell survival.

Lu X, Tang M, Zhu Q, Yang Q, Li Z, Bao Y, Liu G, Hou T, Lv Y, Zhao Y, Wang H, Yang Y, Cheng Z, Wen H, Liu B, Xu X, Gu L, Zhu WG
Nucleic acids research 2019 Dec 2;47(21):10977-10993

---

p62-mediated Selective autophagy endows virus-transformed cells with insusceptibility to DNA damage under oxidative stress.

Wang L, Howell MEA, Sparks-Wallace A, Hawkins C, Nicksic CA, Kohne C, Hall KH, Moorman JP, Yao ZQ, Ning S
PLoS pathogens 2019 Apr;15(4):e1007541

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 21393-1-AP(RNF168 antibody) at dilution of 1:500

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using RNF168 antibody 21393-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line