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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Proximity ligation assay [1]
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Validation data
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- Product number
- H00002243-D01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00002243-D01P, RRID:AB_1573524
- Product name
- FGA purified MaxPab rabbit polyclonal antibody (D01P)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human FGA protein.
- Antigen sequence
MFSMRIVCLVLSVVGTAWTADSGEGDFLAEGGGVR
GPRVVERHQSACKDSDWPFCSGEDWNYKCPSGCRM
KGLIDEVNQDFTNRINKLKNSLFEYQKNNKDSHSL
TTNIMEILRGDFSSANNRDNTYNRVSEDLRSRIEV
LKRKVIEKVTANNLLVARVTTEETPHLKARAIKWQ
MRPEVKPIMKEHIAPREAMLNLALSEVSTLLLWGS
LPCPPRLS- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of FGA expression in transfected 293T cell line (H00002243-T02) by FGA MaxPab polyclonal antibody.Lane 1: FGA transfected lysate(24.70 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- FGA MaxPab rabbit polyclonal antibody. Western Blot analysis of FGA expression in mouse kidney.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of purified MaxPab rabbit antibody to FGA on formalin-fixed paraffin-embedded human ovary. [antibody concentration 1.5 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between FGA and F2. HeLa cells were stained with anti-FGA rabbit purified polyclonal 1:1200 and anti-F2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
