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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP2-50087 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Fibrinogen Antibody
- Antibody type
- Polyclonal
- Description
- Ion exchange chromatography.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Fibrinogen Antibody [NBP2-50087] - Lane 1: Fibrinogen under reducing conditions. Lane 2: none. Load: 1 ug per lane. Primary antibody: Fibrinogen antibody at 1:3000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: TBS/MB-070 1 hr RT.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Fibrinogen Antibody [NBP2-50087] - Tissue: human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Fibrinogen antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase goat secondary antibody at 1:10,000 for 45 min at RT. Localization: Fibrinogen is localized in plasma. Staining: Fibrinogen as precipitated red signal with hematoxylin purple nuclear counterstain.
