LS-C744786

antibody from LSBio

Targeting: FGA

ELISA

Antibody data

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Validation data

Product number: LS-C744786 - Provider product page
Provider: LSBio
Product name: FGA / Fibrinogen Alpha Antibody (Lyophilized, Biotin) LS-C744786
Antibody type: Polyclonal
Description: Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Reactivity: Human
Host: Goat
Conjugate: Biotin
Isotype: IgG
Storage: Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

FGA protein structure - LS-C744786 shown in red.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western Blot of Goat anti-Fibrinogen antibody. Lane 1: Fibrinogen under reducing conditions. Lane 2: none. Load: 1 µg per lane. Primary antibody: Fibrinogen antibody at 1:3000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: TBS/MB-070 1 hr RT.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Immunohistochemistry of Goat Anti-Fibrinogen antibody. Tissue: human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Fibrinogen antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase goat secondary antibody at 1:10,000 for 45 min at RT. Localization: Fibrinogen is localized in plasma. Staining: Fibrinogen as precipitated red signal with hematoxylin purple nuclear counterstain.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Immunohistochemistry of Goat Anti-Fibrinogen antibody. Tissue: human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Fibrinogen antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase goat secondary antibody at 1:10,000 for 45 min at RT. Localization: Fibrinogen is localized in plasma. Staining: Fibrinogen as precipitated red signal with hematoxylin purple nuclear counterstain.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Western Blot of Goat anti-Fibrinogen antibody. Lane 1: Fibrinogen under reducing conditions. Lane 2: none. Load: 1 µg per lane. Primary antibody: Fibrinogen antibody at 1:3000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: TBS/MB-070 1 hr RT.