RQ6053

antibody from NSJ Bioreagents

Targeting: FGA

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ6053

Provider

NSJ Bioreagents

Product name

Fibrinogen alpha chain Antibody (FGA)

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 µg

Storage

After reconstitution, the FGA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) T-47D, 2) Caco-2 and 3) SW620 lysate with FGA antibody. Predicted molecular weight ~70 kDa (alpha isoform), ~95 kDa (alpha-E isoform).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human placenta with FGA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human SiHa cells with FGA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FGA antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HepG2 cells with FGA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FGA antibody.