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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-75234 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NCBP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NCBP2 in paraffin-embedded human breast carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with NCBP2 polyclonal antibody (Product # PA5-75234) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of NCBP2 in HEK293T cell lysate (sepharose bead conjugate), (lane 2 and lane 3) and Nonspecific IgG Control (sepharose bead conjugate, lane 4). Lane 1 is 30% input. Samples were incubated with NCBP2 polyclonal antibody (Product # PA5-75234) Results legends show supernatant (upward arrow) and deposition (downward arrow).
