HPA027288

antibody from Atlas Antibodies

Targeting: POLR3GL flj32422, MGC3200

Western blot (Enhanced validation)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

HPA027288

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA027288, RRID:AB_10602119

Product name

Anti-POLR3GL

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human POLR3GL, Gene description: polymerase (RNA) III (DNA directed) polypeptide G (32kD)-like, Alternative Gene Names: flj32422, MGC3200, Validated applications: WB, IHC, Uniprot ID: Q9BT43, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.5 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

The POLR3G Subunit of Human RNA Polymerase III Regulates Tumorigenesis and Metastasis in Triple-Negative Breast Cancer.

Lautré W, Richard E, Feugeas JP, Dumay-Odelot H, Teichmann M
Cancers 2022 Nov 22;14(23)

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Effects on prostate cancer cells of targeting RNA polymerase III.

Petrie JL, Swan C, Ingram RM, Frame FM, Collins AT, Dumay-Odelot H, Teichmann M, Maitland NJ, White RJ
Nucleic acids research 2019 May 7;47(8):3937-3956

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and POLR3GL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410247).

Sample type

Human

Protocol

Protocol