15417-1-AP

antibody from Proteintech Group

Targeting: BDH1 BDH, SDR9C1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

15417-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#15417-1-AP, RRID:AB_2274683

Product name

BDH1 antibody

Antibody type

Polyclonal

Description

KD/KO validated BDH1 antibody (Cat. #15417-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Combined high-fat, high-sucrose diet and streptozotocin treatment induces cardiometabolic heart failure with preserved ejection fraction in mice.

Heinen A, Spychala A, Ballmann L, Gödecke S, Faradj Z, Bresch F, Krüger M, Bottermann K, Zabri H, Fischer J, Petzsch P, Gödecke A
American journal of physiology. Heart and circulatory physiology 2026 May 1;330(5):H1375-H1390

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β-catenin mutation reprograms ketone body metabolism to drive hepatocellular carcinoma metastasis and resistance to ketogenic therapy via transcriptional activation of OXCT1.

Li H, Qian L, Ji Y, Geng Y, Lu Y, Zhang L, Xu Y, Zong W, Jiang X, Zhou X, Wen J, Liu D, Wang Y, Li Y, Li B, Ma H, Yu D
Cell death & disease 2026 Mar 9;17(1)

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SIRT3 is required for the protective function of ketogenic diet on neural inflammation and neuropathic pain.

Deng M, Fang Y, Chu Y, Ding R, Fan X, Wei H, Song H, Jiang G, Zhang H, Han C, Yuan H
International journal of biological sciences 2025;21(7):3011-3029

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Artesunate promotes intestinal epithelial barrier repair by facilitating HMGCS2-dependent ketogenesis in experimental ulcerative colitis model mice.

Wang G, Han L, Sun H, Sun T, Wu B, Cheng X, Yang S, Zhou D
International immunopharmacology 2025 Sep 23;162:115152

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Dietary lipids, not ketone body metabolites, influence intestinal tumorigenesis in a ketogenic diet.

Shay JES, Chi F, Tzouanas CN, Hoeve JT, Williams KJ, Sever T, Fuentes I, Yilmaz ÖH
bioRxiv : the preprint server for biology 2025 Sep 13;

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β-Hydroxybutyrate enhances malate dehydrogenase 2 β-hydroxybutyrylation to alleviate hepatic steatosis in MASLD.

Cai J, Li W, Wu C, Ni H, Ran H, Huang Y, Tang X, He W, Gu Y, You Y, Li J, Xiao X, Ma L
Cellular and molecular life sciences : CMLS 2025 Oct 24;82(1):361

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The muscle specific MEF2Dα2 isoform promotes muscle ketolysis and running capacity in mice.

Kumar S, Ji X, Iqbal H, Guan X, Mis B, Dave D, Kumar S, Besler J, Dash R, Xia Z, Singh RK
EMBO reports 2025 Nov;26(21):5216-5238

---

The denitrosylase SCoR2 controls cardioprotective metabolic reprogramming.

Grimmett ZW, Zhang R, Zhou HL, Chen Q, Miller D, Qian Z, Lin J, Kalra R, Gross SS, Koch WJ, Premont RT, Stamler JS
eLife 2025 Nov 17;14

---

The denitrosylase SCoR2 controls cardioprotective metabolic reprogramming.

Grimmett ZW, Zhang R, Zhou HL, Chen Q, Miller D, Qian Z, Lin J, Kalra R, Gross SS, Koch WJ, Premont RT, Stamler JS
bioRxiv : the preprint server for biology 2025 Jul 18;

---

Multiplex Cardiac Biomarker Profiling for Precision Coronary Artery Disease Risk Assessment Using SERS with Cubic Core-Satellite Nanostructures.

Zhang T, Li B, Wuethrich A, Liu H, Zhou Q, Hu Z, Wang H, Trau M, Bao F, Li J, Sun Y, Li J
Analytical chemistry 2025 Jul 15;97(27):14750-14760

---

Identification of potential shared core biomarkers in type 2 diabetes and sarcopenia.

Zhang P, Du Y, Zhong X, Wang Y, Pan T
Scientific reports 2025 Jul 15;15(1):25439

---

Acetoacetate ameliorates skin fibrosis by modulating TGF-β1-Smad2/3 signaling pathway.

Shang T, Li L, Fang K, Miao X, Huang J, Jiang Y, Lu W, Cai Z, Lu Y, Cui L, Chu T, Kang H, Li S, Huang J
The Journal of biological chemistry 2025 Dec;301(12):110867

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Constitutive expression of cardiomyocyte Klf9 precipitates metabolic dysfunction and spontaneous cardiomyopathy.

Thakkar C, Alikunju S, Venkatasubramanian A, D'Mello D, Abbas H, Yang Z, Andreas I, Sayed N, Abdellatif M, Sayed D
Cellular signalling 2025 Dec;136:112146

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Oligodendroglial fatty acid metabolism as a central nervous system energy reserve.

Asadollahi E, Trevisiol A, Saab AS, Looser ZJ, Dibaj P, Ebrahimi R, Kusch K, Ruhwedel T, Möbius W, Jahn O, Lee JY, Don AS, Khalil MA, Hiller K, Baes M, Weber B, Abel ED, Ballabio A, Popko B, Kassmann CM, Ehrenreich H, Hirrlinger J, Nave KA
Nature neuroscience 2024 Oct;27(10):1934-1944

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Ketogenic Diet Alters the Epigenetic and Immune Landscape of Prostate Cancer to Overcome Resistance to Immune Checkpoint Blockade Therapy.

Murphy S, Rahmy S, Gan D, Liu G, Zhu Y, Manyak M, Duong L, He J, Schofield JH, Schafer ZT, Li J, Lu X, Lu X
Cancer research 2024 May 15;84(10):1597-1612

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β-hydroxybutyrate restrains colitis-associated tumorigenesis by inhibiting HIF-1α-mediated angiogenesis.

Huang C, Tan H, Wang J, Huang L, Liu H, Shi Y, Zhong C, Weng S, Chen C, Zhao W, Lin Z, Li J, Zhi F, Zhang B
Cancer letters 2024 Jul 1;593:216940

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Ketone flux through BDH1 supports metabolic remodeling of skeletal and cardiac muscles in response to intermittent time-restricted feeding.

Williams AS, Crown SB, Lyons SP, Koves TR, Wilson RJ, Johnson JM, Slentz DH, Kelly DP, Grimsrud PA, Zhang GF, Muoio DM
Cell metabolism 2024 Feb 6;36(2):422-437.e8

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Ketolysis drives CD8(+) T cell effector function through effects on histone acetylation.

Luda KM, Longo J, Kitchen-Goosen SM, Duimstra LR, Ma EH, Watson MJ, Oswald BM, Fu Z, Madaj Z, Kupai A, Dickson BM, DeCamp LM, Dahabieh MS, Compton SE, Teis R, Kaymak I, Lau KH, Kelly DP, Puchalska P, Williams KS, Krawczyk CM, Lévesque D, Boisvert FM, Sheldon RD, Rothbart SB, Crawford PA, Jones RG
Immunity 2023 Sep 12;56(9):2021-2035.e8

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Ketogenic diet modulates cardiac metabolic dysregulation in streptozocin-induced diabetic rats.

Trang NN, Lee TW, Kao YH, Chao TF, Lee TI, Chen YJ
The Journal of nutritional biochemistry 2023 Jan;111:109161

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Loss of hepatic phosphoenolpyruvate carboxykinase 1 dysregulates metabolic responses to acute exercise but enhances adaptations to exercise training in mice.

Rome FI, Shobert GL, Voigt WC, Stagg DB, Puchalska P, Burgess SC, Crawford PA, Hughey CC
American journal of physiology. Endocrinology and metabolism 2023 Jan 1;324(1):E9-E23

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Overcome Prostate Cancer Resistance to Immune Checkpoint Therapy with Ketogenic Diet-Induced Epigenetic Reprogramming.

Murphy S, Rahmy S, Gan D, Zhu Y, Manyak M, Li J, Lu X, Lu X
bioRxiv : the preprint server for biology 2023 Aug 8;

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Novel Prognostic Signatures of Hepatocellular Carcinoma Based on Metabolic Pathway Phenotypes.

Ye T, Lin L, Cao L, Huang W, Wei S, Shan Y, Zhang Z
Frontiers in oncology 2022;12:863266

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Ketogenic diet uncovers differential metabolic plasticity of brain cells.

Düking T, Spieth L, Berghoff SA, Piepkorn L, Schmidke AM, Mitkovski M, Kannaiyan N, Hosang L, Scholz P, Shaib AH, Schneider LV, Hesse D, Ruhwedel T, Sun T, Linhoff L, Trevisiol A, Köhler S, Pastor AM, Misgeld T, Sereda M, Hassouna I, Rossner MJ, Odoardi F, Ischebeck T, de Hoz L, Hirrlinger J, Jahn O, Saher G
Science advances 2022 Sep 16;8(37):eabo7639

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Metabolism-Based Molecular Subtyping Endows Effective Ketogenic Therapy in p53-Mutant Colon Cancer.

Tang M, Xu H, Huang H, Kuang H, Wang C, Li Q, Zhang X, Ge Y, Song M, Zhang X, Wang Z, Ma C, Kang J, Zhang W, Wang Y, Zhang B, Zhang X, Chen Y, Cong M, Melino G, Wang X, Zhou F, Sun Q, Shi H
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022 Oct;9(29):e2201992

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Maternal use of methamphetamine induces sex-dependent changes in myocardial gene expression in adult offspring.

Dague A, Chavva H, Brazeau DA, Denvir J, Rorabaugh BR
Physiological reports 2022 Nov;10(22):e15509

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β-hydroxybutyrate reduces reinstatement of cocaine conditioned place preference through hippocampal CaMKII-α β-hydroxybutyrylation.

Li H, Wan X, Wu Z, Zhou Y, Chen R, Xu W, Zhang J, Yang Z, Bai L, Zhang J, Qin F, Wang L, Chen Y, Jiang L, He Y, Wang X, Wei Q, Li S, Dai Y, Chen Y, Wang Y, Wang H, Tian J, Zhao Y, Cen X
Cell reports 2022 Nov 29;41(9):111724

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Disrupted liver oxidative metabolism in glycine N-methyltransferase-deficient mice is mitigated by dietary methionine restriction.

Rome FI, Hughey CC
Molecular metabolism 2022 Apr;58:101452

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Ketone body oxidation increases cardiac endothelial cell proliferation.

Weis EM, Puchalska P, Nelson AB, Taylor J, Moll I, Hasan SS, Dewenter M, Hagenmüller M, Fleming T, Poschet G, Hotz-Wagenblatt A, Backs J, Crawford PA, Fischer A
EMBO molecular medicine 2022 Apr 7;14(4):e14753

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Ketone body β-hydroxybutyrate ameliorates colitis by promoting M2 macrophage polarization through the STAT6-dependent signaling pathway.

Huang C, Wang J, Liu H, Huang R, Yan X, Song M, Tan G, Zhi F
BMC medicine 2022 Apr 15;20(1):148

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Deficiency of 3-hydroxybutyrate dehydrogenase (BDH1) in mice causes low ketone body levels and fatty liver during fasting.

Otsuka H, Kimura T, Ago Y, Nakama M, Aoyama Y, Abdelkreem E, Matsumoto H, Ohnishi H, Sasai H, Osawa M, Yamaguchi S, Mitchell GA, Fukao T
Journal of inherited metabolic disease 2020 Sep;43(5):960-968

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Hepatocyte-Macrophage Acetoacetate Shuttle Protects against Tissue Fibrosis.

Puchalska P, Martin SE, Huang X, Lengfeld JE, Daniel B, Graham MJ, Han X, Nagy L, Patti GJ, Crawford PA
Cell metabolism 2019 Feb 5;29(2):383-398.e7

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The failing heart utilizes 3-hydroxybutyrate as a metabolic stress defense.

Horton JL, Davidson MT, Kurishima C, Vega RB, Powers JC, Matsuura TR, Petucci C, Lewandowski ED, Crawford PA, Muoio DM, Recchia FA, Kelly DP
JCI insight 2019 Feb 21;4(4)

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Fenofibrate prevents skeletal muscle loss in mice with lung cancer.

Goncalves MD, Hwang SK, Pauli C, Murphy CJ, Cheng Z, Hopkins BD, Wu D, Loughran RM, Emerling BM, Zhang G, Fearon DT, Cantley LC
Proceedings of the National Academy of Sciences of the United States of America 2018 Jan 23;115(4):E743-E752

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Ketone body 3-hydroxybutyrate mimics calorie restriction via the Nrf2 activator, fumarate, in the retina.

Izuta Y, Imada T, Hisamura R, Oonishi E, Nakamura S, Inagaki E, Ito M, Soga T, Tsubota K
Aging cell 2018 Feb;17(1)

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Low ketolytic enzyme levels in tumors predict ketogenic diet responses in cancer cell lines in vitro and in vivo.

Zhang J, Jia PP, Liu QL, Cong MH, Gao Y, Shi HP, Yu WN, Miao MY
Journal of lipid research 2018 Apr;59(4):625-634

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Nutritional Ketosis Affects Metabolism and Behavior in Sprague-Dawley Rats in Both Control and Chronic Stress Environments.

Brownlow ML, Jung SH, Moore RJ, Bechmann N, Jankord R
Frontiers in molecular neuroscience 2017;10:129

---

CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs.

Schatton D, Pla-Martin D, Marx MC, Hansen H, Mourier A, Nemazanyy I, Pessia A, Zentis P, Corona T, Kondylis V, Barth E, Schauss AC, Velagapudi V, Rugarli EI
The Journal of cell biology 2017 Mar 6;216(3):675-693

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Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.

Huang D, Li T, Wang L, Zhang L, Yan R, Li K, Xing S, Wu G, Hu L, Jia W, Lin SC, Dang CV, Song L, Gao P, Zhang H
Cell research 2016 Oct;26(10):1112-1130

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Impairments of hepatic gluconeogenesis and ketogenesis in PPARα-deficient neonatal mice.

Cotter DG, Ercal B, d'Avignon DA, Dietzen DJ, Crawford PA
American journal of physiology. Endocrinology and metabolism 2014 Jul 15;307(2):E176-85

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Impact of peripheral ketolytic deficiency on hepatic ketogenesis and gluconeogenesis during the transition to birth.

Cotter DG, Ercal B, d'Avignon DA, Dietzen DJ, Crawford PA
The Journal of biological chemistry 2013 Jul 5;288(27):19739-49

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

human liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP(BDH1 antibody) at dilution of 1:500

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver using 15417-1-AP(BDH1 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The BDH1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human BDH1. This antibody recognizes human,mouse,rat antigen. The BDH1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

15417-1-AP.pdf

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