MA5-15594

antibody from Invitrogen Antibodies

Targeting: BDH1 BDH, SDR9C1

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15594

Provider

Invitrogen Antibodies

Product name

BDH1 Monoclonal Antibody (1A5)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15594 targets BDH1 in indirect ELISA, IHC and WB applications and shows reactivity with Human and mouse samples. The MA5-15594 immunogen is purified recombinant fragment of human BDH1 expressed in E. Coli. MA5-15594 detects BDH1 which has a predicted molecular weight of approximately 38kDa.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

1A5

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

E2F6 Impairs Glycolysis and Activates BDH1 Expression Prior to Dilated Cardiomyopathy.

Major JL, Dewan A, Salih M, Leddy JJ, Tuana BS
PloS one 2017;12(1):e0170066

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 1 E2F6 Activates BDH1 Expression in Neonatal Myocardium. ( A) Bdh1 (beta-hydroxybutyrate dehydrogenase 1) and Oxct1 (succinyl-CoA: 3-oxoacid-CoA transferase) transcript levels in Wt and Tg myocardium 7 days after birth. Expression is normalized to Gapdh . Results represent mean+-SEM values (n = 5-7). ( B) Representative immunoblots of protein from Wt and Tg mouse myocardium at post-natal day 1 (pups) and 6wks of age (adult) examined with the anti-BDH1 and anti-OXCT1. ( C) Densitometric quantification of BDH1 and OXCT1 expression from immunoblots. Expression is normalized against GAPDH. Results represent mean+-SEM values (n = 4). ( D) Immunoblot of protein from Wt and Tg myocardium with BDH1 at the indicated time points after birth.* P

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 5 beta-OHB Regulates CX-43 Protein Expression in Neonatal Cardiomyocytes. ( A) Representative immunoblot of protein isolated from Wt and Tg myocardium at post-natal day 1 with anti-CX-43. ( B) Densitometric quantification of CX-43 immunoblot. Expression is normalized against GAPDH. Results represent mean+-SEM values (n = 4). ( C) Representative immunoblot analyses of protein from Wt and Tg neonatal cardiomyocytes following incubation with or without beta-OHB (beta-hydroxybutyrate). ( D) Densitometric quantification of CX-43 in treated and non-treated neonatal cardiomyocytes. Expression is normalized against GAPDH. Results represent the mean+-SEM values (n = 3-5). A two-way ANOVA was used to measure statistical significance. ( E ) Representative immunoblot analysis of BDH1 in Wt and Tg neonatal cardiomyocytes. * P