HPA027516

antibody from Atlas Antibodies

Targeting: POLR3C C82, RPC3, RPC62

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

HPA027516

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA027516, RRID:AB_10600894

Product name

Anti-POLR3C

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human POLR3C, Gene description: polymerase (RNA) III (DNA directed) polypeptide C (62kD), Alternative Gene Names: RPC3, RPC62, Validated applications: WB, ICC, Uniprot ID: Q9BUI4, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.1 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Inborn errors in RNA polymerase III underlie severe varicella zoster virus infections

Ogunjimi B, Zhang S, Sørensen K, Skipper K, Carter-Timofte M, Kerner G, Luecke S, Prabakaran T, Cai Y, Meester J, Bartholomeus E, Bolar N, Vandeweyer G, Claes C, Sillis Y, Lorenzo L, Fiorenza R, Boucherit S, Dielman C, Heynderickx S, Elias G, Kurotova A, Auwera A, Verstraete L, Lagae L, Verhelst H, Jansen A, Ramet J, Suls A, Smits E, Ceulemans B, Van Laer L, Plat Wilson G, Kreth J, Picard C, Von Bernuth H, Fluss J, Chabrier S, Abel L, Mortier G, Fribourg S, Mikkelsen J, Casanova J, Paludan S, Mogensen T
Journal of Clinical Investigation 2017;127(9):3543-3556

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and POLR3C over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401943).

Sample type

Human

Protocol

Protocol