Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 53-1609-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD160 Monoclonal Antibody (BY55), Alexa Fluor 488, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This BY55 monoclonal antibody reacts with human CD160, a member of the immunoglobulin superfamily expressed on natural killer cells, natural killer T cells, activated and/or memory CD4 and CD8 T cells, gamma/delta T cells, and intraepithelial cells. CD160 has been reported to bind herpes virus entry mediator (HVEM) in a complex with LIGHT and BTLA to deliver an inhibitory signal to CD4 T cells that abrogates proliferation and cytokine production. Three alternative splice variants of CD160 have also been identified in natural killer cells. Applications Reported: This BY55 antibody has been reported for use in flow cytometric analysis. Applications Tested: This BY55 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgM
- Antibody clone number
- BY55
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references OMIP-037: 16-color panel to measure inhibitory receptor signatures from multiple human immune cell subsets.
CD4+ T Cells Expressing PD-1, TIGIT and LAG-3 Contribute to HIV Persistence during ART.
Liver environment and HCV replication affect human T-cell phenotype and expression of inhibitory receptors.
The CD160, BTLA, LIGHT/HVEM pathway: a bidirectional switch regulating T-cell activation.
Identification and characterization of a transmembrane isoform of CD160 (CD160-TM), a unique activating receptor selectively expressed upon human NK cell activation.
CD160 inhibits activation of human CD4+ T cells through interaction with herpesvirus entry mediator.
BY55 monoclonal antibody delineates within human cord blood and bone marrow lymphocytes distinct cell subsets mediating cytotoxic activity.
Belkina AC, Snyder-Cappione JE
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2017 Feb;91(2):175-179
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2017 Feb;91(2):175-179
CD4+ T Cells Expressing PD-1, TIGIT and LAG-3 Contribute to HIV Persistence during ART.
Fromentin R, Bakeman W, Lawani MB, Khoury G, Hartogensis W, DaFonseca S, Killian M, Epling L, Hoh R, Sinclair E, Hecht FM, Bacchetti P, Deeks SG, Lewin SR, Sékaly RP, Chomont N
PLoS pathogens 2016 Jul;12(7):e1005761
PLoS pathogens 2016 Jul;12(7):e1005761
Liver environment and HCV replication affect human T-cell phenotype and expression of inhibitory receptors.
Kroy DC, Ciuffreda D, Cooperrider JH, Tomlinson M, Hauck GD, Aneja J, Berger C, Wolski D, Carrington M, Wherry EJ, Chung RT, Tanabe KK, Elias N, Freeman GJ, de Kruyff RH, Misdraji J, Kim AY, Lauer GM
Gastroenterology 2014 Feb;146(2):550-61
Gastroenterology 2014 Feb;146(2):550-61
The CD160, BTLA, LIGHT/HVEM pathway: a bidirectional switch regulating T-cell activation.
Cai G, Freeman GJ
Immunological reviews 2009 May;229(1):244-58
Immunological reviews 2009 May;229(1):244-58
Identification and characterization of a transmembrane isoform of CD160 (CD160-TM), a unique activating receptor selectively expressed upon human NK cell activation.
Giustiniani J, Bensussan A, Marie-Cardine A
Journal of immunology (Baltimore, Md. : 1950) 2009 Jan 1;182(1):63-71
Journal of immunology (Baltimore, Md. : 1950) 2009 Jan 1;182(1):63-71
CD160 inhibits activation of human CD4+ T cells through interaction with herpesvirus entry mediator.
Cai G, Anumanthan A, Brown JA, Greenfield EA, Zhu B, Freeman GJ
Nature immunology 2008 Feb;9(2):176-85
Nature immunology 2008 Feb;9(2):176-85
BY55 monoclonal antibody delineates within human cord blood and bone marrow lymphocytes distinct cell subsets mediating cytotoxic activity.
Bensussan A, Gluckman E, el Marsafy S, Schiavon V, Mansur IG, Dausset J, Boumsell L, Carosella E
Proceedings of the National Academy of Sciences of the United States of America 1994 Sep 13;91(19):9136-40
Proceedings of the National Academy of Sciences of the United States of America 1994 Sep 13;91(19):9136-40
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD3 PE (Product # 12-0037-42) and Anti-Human CD160 Alexa Fluor® 488 (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Green dye