33-4300

Fabbiani G, Reali C, Valentín-Kahan A, Rehermann MI, Fagetti J, Falco MV, Russo RE
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Mar 11;40(11):2246-2258

Cholesterol metabolism and Cx43, Cx46, and Cx50 gap junction protein expression and localization in normal and diabetic and obese ob/ob and db/db mouse testes.

Pelletier RM, Akpovi CD, Chen L, Vitale ML
American journal of physiology. Endocrinology and metabolism 2018 Jan 1;314(1):E21-E38

Expression and Localization of Connexins in the Outer Retina of the Mouse.

Bolte P, Herrling R, Dorgau B, Schultz K, Feigenspan A, Weiler R, Dedek K, Janssen-Bienhold U
Journal of molecular neuroscience : MN 2016 Feb;58(2):178-92

Connexin 50 modulates Sox2 expression in spinal-cord-derived ependymal stem/progenitor cells.

Rodriguez-Jimenez FJ, Alastrue A, Stojkovic M, Erceg S, Moreno-Manzano V
Cell and tissue research 2016 Aug;365(2):295-307

Connexin50 couples axon terminals of mouse horizontal cells by homotypic gap junctions.

Dorgau B, Herrling R, Schultz K, Greb H, Segelken J, Ströh S, Bolte P, Weiler R, Dedek K, Janssen-Bienhold U
The Journal of comparative neurology 2015 Oct 1;523(14):2062-81

Hyposmotic stress causes ATP release and stimulates Na,K-ATPase activity in porcine lens.

Shahidullah M, Mandal A, Beimgraben C, Delamere NA
Journal of cellular physiology 2012 Apr;227(4):1428-37

PKCγ, role in lens differentiation and gap junction coupling.

Das S, Wang H, Molina SA, Martinez-Wittinghan FJ, Jena S, Bossmann LK, Miller KA, Mathias RT, Takemoto DJ
Current eye research 2011 Jul;36(7):620-31

Gap junctional coupling and connexin immunoreactivity in rabbit retinal glia.

Zahs KR, Ceelen PW
Visual neuroscience 2006 Jan-Feb;23(1):1-10

Expression of connexins in chromaffin cells of normal human adrenals and in benign and malignant pheochromocytomas.

Willenberg HS, Schott M, Saeger W, Tries A, Scherbaum WA, Bornstein SR
Annals of the New York Academy of Sciences 2006 Aug;1073:578-83

Regulation of lens cell-to-cell communication by activation of PKCgamma and disassembly of Cx50 channels.

Zampighi GA, Planells AM, Lin D, Takemoto D
Investigative ophthalmology & visual science 2005 Sep;46(9):3247-55

Differential phosphorylation of connexin46 and connexin50 by H2O2 activation of protein kinase Cgamma.

Lin D, Lobell S, Jewell A, Takemoto DJ
Molecular vision 2004 Sep 27;10:688-95

Growth factors but not gap junctions play a role in injury-induced Ca2+ waves in epithelial cells.

Klepeis VE, Cornell-Bell A, Trinkaus-Randall V
Journal of cell science 2001 Dec;114(Pt 23):4185-95

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Connexin 50 was performed by loading 20 µg of Rat Brain (lane1) and Mouse Brain (lane2) lysates using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. Connexin 50 was detected at ~48 kDa using Connexin 50 Mouse Monoclonal Antibody (Product # 33-4300) at 0.5-1 µg/mL in 2.5 % skim milk at 4°C overnight on a rocking platform. Goat Anti-Mouse IgG - HRP Secondary Antibody (Product # 62-6520) at 1:4000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Connexin 50 was done on 90% confluent log phase CaCo-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with Connexin 50 Mouse Monoclonal Antibody (Product # 33-4300) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (Product # A12381). Panel d is a merged image showing junctional localization of Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059). and panel e is a no primary antibody control. The images were captured at 20X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL