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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-56637 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZC3H15 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: IVCKHFLEAI ENNKYGWFWV CPGGGDICMY RHALPPGFVL KKDKKKEEKE DEISLEDLIE RERSALGPNV TKITL Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 97%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.42 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZC3H15 in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a ZC3H15 Polyclonal Antibody (Product # PA5-56637). Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZC3H15 in control (vector only transfected HEK293T lysate) and ZC3H15 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a ZC3H15 Polyclonal Antibody (Product # PA5-56637).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ZC3H15 in human cell line U-2 OS shows positivity in cytoplasm. Samples were probed using a ZC3H15 Polyclonal Antibody (Product # PA5-56637).
