15857-1-AP

antibody from Proteintech Group

Targeting: H2BC21 H2B, H2B.1, H2B/q, H2BE, H2BFQ, HIST2H2BE

Provider product page for 15857-1-AP

Western blot

ELISA

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [29]
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Validations
Immunohistochemistry [2]

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Product number: 15857-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#15857-1-AP, RRID:AB_10664929
Product name: Histone H2B antibody
Antibody type: Polyclonal
Description: Histone H2B antibody (Cat. #15857-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

H2BC21 protein structure - 15857-1-AP shown in red.

Submitted references NPM3 functions as a lactyltransferase to promote necroptosis in male diabetic cardiomyopathy mice models via FASN transcription modulation.

Xu H, Jiang X, Wang F, Meng F, Liu Y, Huang J, Li J, Zhu M
Nature communications 2026 Mar 9;17(1)

Parvimonas micra-derived SirTM: An ADP-ribosyltransferase as a novel driver in colorectal cancer progression.

Chang Y, Yang C, Hou F, Zhu Y, Huang Z, Wang L, Liu Y, Zhang H, Qin X, Hao Z, Li D, Tan Y, Bai Y, Ding L, Gao H, Zhi F, Zhang F, Bi Y, Yang R
Journal of advanced research 2026 Mar 17;

Lactate Prevents Glucose Deprivation-Induced Parthanatos in Gastric Cancer Cells Through Alleviating Intracellular Reactive Oxygen Species.

Niu D, Xia Y, Qin X, Zhang M, Pan S, Yuan X, Tang G, Ai W, Liang Y, Li Z, Xie Z
Cell biology international 2025 Nov;49(11):1441-1453

Histone Lactylation-Driven Upregulation of VRK1 Expression Promotes Stemness and Proliferation of Glioma Stem Cells.

Li J, Tang C, Zhang X, Xing R, Guo Q
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Nov;12(44):e03897

Co-inhibition of Notch1 and ChK1 triggers genomic instability and melanoma cell death increasing the lifespan of mice bearing melanoma brain metastasis.

Thakur V, Thakur VS, Wang D, de Freitas JT, Bianchi A, Nivelo LA, Umland O, Welford SM, Bedogni B
Journal of experimental & clinical cancer research : CR 2025 May 28;44(1):163

Epigenetic dynamics in meniscus cell migration and its zonal dependency in response to inflammatory conditions.

Zhang Y, Zhang EY, Cheung C, Heo Y, Tumenbayar BI, Lee SH, Bae Y, Heo SC
APL bioengineering 2025 Mar;9(1):016109

Epigenetic regulation and mechanobiological adaptation in tenocytes during maturation.

Zhang EY, Blanch TE, Ahmed SB, Jiang X, Dyment NA, Heo SC
APL bioengineering 2025 Jun;9(2):026127

O-SNAP: A comprehensive pipeline for spatial profiling of chromatin architecture.

Kim HH, Martinez-Sarmiento JA, Palma FR, Kant A, Zhang EY, Guo Z, Mauck RL, Heo SC, Shenoy V, Bonini MG, Lakadamyali M
bioRxiv : the preprint server for biology 2025 Jul 23;

Wnt signaling modulates mechanotransduction in the epidermis to drive hair follicle regeneration.

Oak ASW, Bagchi A, Brukman MJ, Toth J, Ford J, Zheng Y, Nace A, Yang R, Hsieh JC, Hayden JE, Ruthel G, Ray A, Kim E, Shenoy VB, Cotsarelis G
Science advances 2025 Feb 21;11(8):eadq0638

Revealing the biophysics of lamina-associated domain formation by integrating theoretical modeling and high-resolution imaging.

Dhankhar M, Guo Z, Kant A, Basir R, Joshi R, Vinayak V, Heo SC, Mauck RL, Lakadamyali M, Shenoy VB
Nature communications 2025 Aug 25;16(1):7909

Polymer model integrates imaging and sequencing to reveal how nanoscale heterochromatin domains influence gene expression.

Vinayak V, Basir R, Golloshi R, Toth J, Sant'Anna L, Lakadamyali M, McCord RP, Shenoy VB
Nature communications 2025 Apr 23;16(1):3816

Nuclear proteasomes buffer cytoplasmic proteins during autophagy compromise.

Park SJ, Son SM, Barbosa AD, Wrobel L, Stamatakou E, Squitieri F, Balmus G, Rubinsztein DC
Nature cell biology 2024 Oct;26(10):1691-1699

Dissecting gene activation and chromatin remodeling dynamics in single human cells undergoing reprogramming.

Martinez-Sarmiento JA, Cosma MP, Lakadamyali M
Cell reports 2024 May 28;43(5):114170

Revealing the Biophysics of Lamina-Associated Domain Formation by Integrating Theoretical Modeling and High-Resolution Imaging.

Dhankhar M, Guo Z, Kant A, Basir R, Joshi R, Heo SC, Mauck RL, Lakadamyali M, Shenoy VB
bioRxiv : the preprint server for biology 2024 Jun 25;

Epigenetic Dynamics in Meniscus Cell Migration and its Zonal Dependency in Response to Inflammatory Conditions: Implications for Regeneration Strategies.

Zhang Y, Zhang Y, Wang C, Heo Y, Tumenbayar BI, Lee SH, Bae Y, Chin Heo S
bioRxiv : the preprint server for biology 2024 Jul 23;

Aberrant chromatin reorganization in cells from diseased fibrous connective tissue in response to altered chemomechanical cues.

Heo SJ, Thakur S, Chen X, Loebel C, Xia B, McBeath R, Burdick JA, Shenoy VB, Mauck RL, Lakadamyali M
Nature biomedical engineering 2023 Feb;7(2):177-191

Cancer-associated fibroblasts require proline synthesis by PYCR1 for the deposition of pro-tumorigenic extracellular matrix.

Kay EJ, Paterson K, Riera-Domingo C, Sumpton D, Däbritz JHM, Tardito S, Boldrini C, Hernandez-Fernaud JR, Athineos D, Dhayade S, Stepanova E, Gjerga E, Neilson LJ, Lilla S, Hedley A, Koulouras G, McGregor G, Jamieson C, Johnson RM, Park M, Kirschner K, Miller C, Kamphorst JJ, Loayza-Puch F, Saez-Rodriguez J, Mazzone M, Blyth K, Zagnoni M, Zanivan S
Nature metabolism 2022 Jun;4(6):693-710

Histone methyltransferase Dot1L recruits O-GlcNAc transferase to target chromatin sites to regulate histone O-GlcNAcylation.

Xu B, Zhang C, Jiang A, Zhang X, Liang F, Wang X, Li D, Liu C, Liu X, Xia J, Li Y, Wang Y, Yang Z, Chen J, Zhou Y, Chen L, Sun H
The Journal of biological chemistry 2022 Jul;298(7):102115

Intercellular nanotubes mediate mitochondrial trafficking between cancer and immune cells.

Saha T, Dash C, Jayabalan R, Khiste S, Kulkarni A, Kurmi K, Mondal J, Majumder PK, Bardia A, Jang HL, Sengupta S
Nature nanotechnology 2022 Jan;17(1):98-106

Non-canonical H3K79me2-dependent pathways promote the survival of MLL-rearranged leukemia.

Richter WF, Shah RN, Ruthenburg AJ
eLife 2021 Jul 15;10

USP7 negatively controls global DNA methylation by attenuating ubiquitinated histone-dependent DNMT1 recruitment.

Li J, Wang R, Jin J, Han M, Chen Z, Gao Y, Hu X, Zhu H, Gao H, Lu K, Shao Y, Lyu C, Lai W, Li P, Hu G, Li J, Li D, Wang H, Wu Q, Wong J
Cell discovery 2020;6:58

RAD6B Plays a Critical Role in Neuronal DNA Damage Response to Resist Neurodegeneration.

Guo Z, Tian Y, Guo Y, Li B, Liu X, Xie K, Song Y, Wang D
Frontiers in cellular neuroscience 2019;13:392

In the human sperm nucleus, nucleosomes form spatially restricted domains consistent with programmed nucleosome positioning.

Zhang MZ, Cao XM, Xu FQ, Liang XW, Fu LL, Li B, Liu WG, Li SG, Sun FZ, Huang XY, Huang WH
Biology open 2019 Jul 10;8(7)

Function of RAD6B and RNF8 in spermatogenesis.

Guo Y, Song Y, Guo Z, Hu M, Liu B, Duan H, Wang L, Yuan T, Wang D
Cell cycle (Georgetown, Tex.) 2018;17(2):162-173

HIPK2 and extrachromosomal histone H2B are separately recruited by Aurora-B for cytokinesis.

Monteonofrio L, Valente D, Ferrara M, Camerini S, Miscione R, Crescenzi M, Rinaldo C, Soddu S
Oncogene 2018 Jun;37(26):3562-3574

Chemoproteomics Reveals Chemical Diversity and Dynamics of 4-Oxo-2-nonenal Modifications in Cells.

Sun R, Fu L, Liu K, Tian C, Yang Y, Tallman KA, Porter NA, Liebler DC, Yang J
Molecular & cellular proteomics : MCP 2017 Oct;16(10):1789-1800

Highly Selective and Large Scale Mass Spectrometric Analysis of 4-Hydroxynonenal Modification via Fluorous Derivatization and Fluorous Solid-Phase Extraction.

Yuan W, Zhang Y, Xiong Y, Tao T, Wang Y, Yao J, Zhang L, Yan G, Bao H, Lu H
Analytical chemistry 2017 Mar 7;89(5):3093-3100

Regulation of the RhoA/ROCK/AKT/β-catenin pathway by arginine-specific ADP-ribosytransferases 1 promotes migration and epithelial-mesenchymal transition in colon carcinoma.

Song GL, Jin CC, Zhao W, Tang Y, Wang YL, Li M, Xiao M, Li X, Li QS, Lin X, Chen WW, Kuang J
International journal of oncology 2016 Aug;49(2):646-56

Histone monoubiquitylation position determines specificity and direction of enzymatic cross-talk with histone methyltransferases Dot1L and PRC2.

Whitcomb SJ, Fierz B, McGinty RK, Holt M, Ito T, Muir TW, Allis CD
The Journal of biological chemistry 2012 Jul 6;287(28):23718-25

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human pancreas using 15857-1-AP(HIST2H2BE antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The Histone H2B antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human Histone H2B. This antibody recognizes human antigen. The Histone H2B antibody has been validated for the following applications: ELISA, IHC analysis.
Appendix: 15857-1-AP.pdf

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