PA5-80687
antibody from Invitrogen Antibodies
Targeting: H2BC21
H2B, H2B.1, H2B/q, H2BE, H2BFQ, HIST2H2BE
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-80687 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HIST2H2BE Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HIST2H2BE in Lane A: Jurkat Whole Cell Lysate (30 µg). Samples were probed using a HIST2H2BE Polyclonal Antibody (Product # PA5-80687) at a 1:1000 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:14 kDa. Observed band size:15 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HIST2H2BE Polyclonal Antibody (Product # PA5-80687) at 1:1,000 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 15 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human HIST2H2BE in human breast carcinoma with HIST2H2BE Polyclonal Antibody (Product # PA5-80687, 1:20,000, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human HIST2H2BE in human kidney with HIST2H2BE Polyclonal Antibody (Product # PA5-80687, 1:20,000, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human HIST2H2BE in human testis with HIST2H2BE Polyclonal Antibody (Product # PA5-80687, 1:20,000, formalin-fixed paraffin embedded sections).