AP3468a

antibody from Abcepta

Targeting: AKT3 PKBG, PRKBG, RAC-gamma

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AP3468a

Provider

Abcepta

Proper citation

Abgent Cat#AP3468a, RRID:AB_2225499

Product name

Phospho-AKT3(S472) Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

Submitted references

Blockade of CCN6 (WISP3) activates growth factor-independent survival and resistance to anoikis in human mammary epithelial cells.

Huang W, Gonzalez ME, Toy KA, Banerjee M, Kleer CG
Cancer research 2010 Apr 15;70(8):3340-50

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Akt1 mediates purinergic-dependent NOS3 activation in thick ascending limbs.

Silva GB, Garvin JL
American journal of physiology. Renal physiology 2009 Sep;297(3):F646-52

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Dot blot analysis of anti-AKT3-pS472 Phospho-specific Pab (RB13331) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Primary Ab dilution

1:500