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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA500001 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500001, RRID:AB_2305368
- Product name
- AKT3 mouse monoclonal antibody, clone OTI9B2 (formerly 9B2)
- Antibody type
- Monoclonal
- Description
- AKT3 mouse monoclonal antibody, clone OTI9B2 (formerly 9B2)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- AKT3
- Isotype
- IgG
- Antibody clone number
- OTI9B2
- Vial size
- 100 µl
- Concentration
- 0.25 mg/ml
Submitted references Regulation of the PI3-K/Akt survival pathway in the rat endometrium.
Veillette A, Grenier K, Brasseur K, Fréchette-Frigon G, Leblanc V, Parent S, Asselin E
Biology of reproduction 2013 Mar;88(3):79
Biology of reproduction 2013 Mar;88(3):79
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- AKT3 antibody ( 9B2 ) at 1:500 dilution + BALB/C 3T3 cell lysate
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3 (RC221051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-AKT3 monoclonal antibody at 1:200 dilution.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Western Blot of Akt3 protein level by using anti-Akt3 antibody in rat endometrium during pregnancy and pseudopregnancy.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-AKT3 mouse monoclonal antibody (TA500001) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT3(RC221051).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-AKT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500001)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC221051 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AKT3 antibody(TA500001), and then analyzed by flow cytometry.
- Validation comment
- FC
