R33341

antibody from NSJ Bioreagents

Targeting: AKT3 PKBG, PRKBG, RAC-gamma

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R33341

Provider

NSJ Bioreagents

Product name

AKT3 Antibody

Antibody type

Polyclonal

Description

This highly specific AKT3 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/Immunofluorescence/ELISA applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Goat

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide

Storage

Aliquot and store the AKT3 antibody at -20oC.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of HepG2 [A], Jurkat [B], mouse brain [C] and rat brain [D] lysates with AKT3 antibody at 1ug/ml. Predicted molecular weight: ~56kDa but can be observed from 60~65kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot of HEK293 lysate overexpressing AKT3 probed with AKT3 antibody (mock transfection on right). Predicted molecular weight: ~56kDa but can be observed from 60~65kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of HepG2 [A], Jurkat [B], mouse brain [C] and rat brain [D] lysates with AKT3 antibody at 1ug/ml. Predicted molecular weight: ~56kDa but can be observed from 60~65kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot of 1A) human thyroid, 2) mouse brain and 3) rat brain tissue lysate probed with AKT3 antibody. Predicted molecular weight: ~56 kDa but can be observed from 60~65 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IF/ICC staining of fixed and permeabilized human ThP-1 cells with AKT3 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IF/ICC staining of fixed and permeabilized human A431 cells with AKT3 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human prostate tissue with AKT3 antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human heart tissue with AKT3 antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human prostate tissue with AKT3 antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human heart tissue with AKT3 antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

FACS testing of fixed and permeabilized human HepG2 cells with AKT3 antibody (blue) at 10ug/ml and naive goat Ig (black).