Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- R33341 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- AKT3 Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids CSPTSQIDNIGEEEM were used as the immunogen for this AKT3 antibody. (1)
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store the AKT3 antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of HepG2 [A], Jurkat [B], mouse brain [C] and rat brain [D] lysates with AKT3 antibody at 1ug/ml. Predicted molecular weight: ~56kDa but can be observed from 60~65kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot of HEK293 lysate overexpressing AKT3 probed with AKT3 antibody (mock transfection on right). Predicted molecular weight: ~56kDa but can be observed from 60~65kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human prostate tissue with AKT3 antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human heart tissue with AKT3 antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.