R31321

antibody from NSJ Bioreagents

Targeting: AKT3 PKBG, PRKBG, RAC-gamma

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R31321

Provider

NSJ Bioreagents

Product name

AKT3 Antibody

Antibody type

Polyclonal

Antigen

Amino acids 122-136 (TSQIDNIGEEEMDAS) were used as the immunogen for this AKT3 antibody (100% homologous in human, mouse and rat).

Description

Antigen affinity purified antibody

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 µg

Concentration

Lyophilized; resuspend with 200 ul for 0.5 mg/ml

Storage

After reconstitution, the AKT3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of AKT3 antibody and Lane 1: rat lung; 2: (r) kidney; 3: human HeLa; 4: (h) placenta; 5: (h) A549; 6: mouse NIH3T3 cell lysate. Expected/observed size 56~70KD

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ICC testing of AKT3 antibody and mouse HEPA cells

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: AKT3 antibody testing of human breast cancer tissue. HIER: steamed with pH6 citrate buffer.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P testing of rat kidney tissue. HIER: steamed with pH6 citrate buffer.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P testing of rat lung tissue. HIER: steamed with pH6 citrate buffer.