Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- F00520 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-AKT3 FITC Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal 25F6.F6.D8 IgG1 kappa antibody for AKT3 detection. Host: Mouse.Size: 50μg. Tested applications: IHC. Reactive species: Human. AKT3 information: Molecular Weight: 55775 MW; Subcellular Localization: Nucleus . Cytoplasm . Membrane ; Peripheral membrane protein . Membrane-associated after cell stimulation leading to its translocation; Tissue Specificity: In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Antibody clone number
- 25F6.F6.D8 IgG1 kappa
- Vial size
- 50μg
- Concentration
- 1.0 mg/mL by UV absorbance at 280 nm
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of AKT3 expression in GST Tagged recombinant AKT1 (lane 1), GST Tagged recombinant AKT2 (lane 2) and GST Tagged recombinant AKT3 (lane 3). AKT3 at 78KD was detected using mouse anti-AKT3 unconjugated monoclonal antibody at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).
- Additional image