LS-C744847

antibody from LSBio

Targeting: AKT3 PKBG, PRKBG, RAC-gamma

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C744847

Provider

LSBio

Product name

AKT3 Antibody (Internal, clone 25F6.F6.D8) LS-C744847

Antibody type

Monoclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

25F6.F6.D8

Storage

Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot of Mouse anti-AKT3 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT3 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:40,000 for 30 min at RT.