LS-C744901

antibody from LSBio

Targeting: AKT3 PKBG, PRKBG, RAC-gamma

Western blot (Data presented on provider website)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C744901

Provider

LSBio

Product name

AKT3 Antibody (Internal, clone 9A5.H9.G7) LS-C744901

Antibody type

Monoclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

9A5.H9.G7

Storage

Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

ELISA

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

ELISA of Mouse Monoclonal anti-AKT3 antibody. Antigen: GST AKT1, GST AKT2, GST AKT3. Coating amount: starting from 50 ng/well. Primary antibody: Mouse monoclonal anti-AKT3 antibody at 100ng/well. Dilution series: 2-fold. Mid-point concentration: 3 ng/mL Mouse monoclonal anti-AKT3 antibody. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ELISA of Mouse Monoclonal anti-AKT3 antibody. Antigen: GST AKT1, GST AKT2, GST AKT3. Coating amount: starting from 50 ng/well. Primary antibody: Mouse monoclonal anti-AKT3 antibody at 100ng/well. Dilution series: 2-fold. Mid-point concentration: 3 ng/mL Mouse monoclonal anti-AKT3 antibody. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB