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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NBP1-47657 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-47657, RRID:AB_10010610
- Product name
- Mouse Monoclonal AKT3 Antibody
- Antibody type
- Monoclonal
- Description
- Affinity purified. This antibody is specific for Homo sapiens v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) (AKT3), transcript variant 1.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.25 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AKT3 Antibody (9B2) [NBP1-47657] - AKT3 antibody ( 9B2 ) at 1:500 dilution + BALB/C 3T3 cell lysate
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AKT3 Antibody (9B2) [NBP1-47657] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AKT3 Antibody (OTI9B2) [NBP1-47657] - Analysis of extracts (10ug) from 2 different cell lines by using anti-AKT3 monoclonal antibody at 1:200 dilution.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: AKT3 Antibody (9B2) [NBP1-47657] - Staining of paraffin-embedded Human lung tissue using anti-AKT3 mouse monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: AKT3 Antibody (9B2) [NBP1-47657] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AKT3 antibody, and then analyzed by flow cytometry.
