Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP1-80900 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-80900, RRID:AB_11037862
- Product name
- Rabbit Polyclonal AKT3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse AKT3 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Investigation of molecular alterations of AKT-3 in triple-negative breast cancer.
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Abrogation of BRAFV600E-induced senescence by PI3K pathway activation contributes to melanomagenesis.
O'Hurley G, Daly E, O'Grady A, Cummins R, Quinn C, Flanagan L, Pierce A, Fan Y, Lynn MA, Rafferty M, Fitzgerald D, Pontén F, Duffy MJ, Jirström K, Kay EW, Gallagher WM
Histopathology 2014 Apr;64(5):660-70
Histopathology 2014 Apr;64(5):660-70
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23
Nature methods 2013 Apr;10(4):315-23
Abrogation of BRAFV600E-induced senescence by PI3K pathway activation contributes to melanomagenesis.
Vredeveld LC, Possik PA, Smit MA, Meissl K, Michaloglou C, Horlings HM, Ajouaou A, Kortman PC, Dankort D, McMahon M, Mooi WJ, Peeper DS
Genes & development 2012 May 15;26(10):1055-69
Genes & development 2012 May 15;26(10):1055-69
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AKT3 Antibody [NBP1-80900] - Analysis in human cell line SH-SY5Y.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: AKT3 Antibody [NBP1-80900] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: AKT3 Antibody [NBP1-80900] - Imaging of mouse spinal cord (DAB). This image was submitted via customer Review.